Helicobacter pylori apo-Fur regulation appears unconserved across species.
Helicobacter pylori apo-Fur regulation appears unconserved across species.
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DOI：
10.1007/s12275-010-0022-0
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发表时间：
2010-06
影响因子：
3
通讯作者：
Merrell, D. Scott
中科院分区：
文献类型：
--
作者：
Miles, Shana;Carpenter, Beth M.;Gancz, Hanan;Merrell, D. Scott
铁吸收调节因子（Fur）是一种转录调节因子，在许多细菌物种中保守，并已被证明可以调节铁吸收和储存基因的表达。此外，Fur已被证明是胃病原体幽门螺杆菌的重要定植因子。In H. pylori中，Fur依赖性调节似乎是独特的，因为Fur在与铁结合时以及在其无铁（apo）形式下能够充当转录阻遏物。到目前为止，载脂蛋白调节尚未确定在任何其他细菌。为了确定来自其他物种的毛皮是否具有apo调节的能力，我们研究了来自大肠杆菌、空肠弯曲杆菌、脱硫弧菌、铜绿假单胞菌和霍乱弧菌的毛皮在H. pylori fur突变体。我们发现，虽然一些毛皮物种（E。coli、C.空肠弧菌和霍乱弧菌）补充铁结合的调节，载脂蛋白调节不能被任何检查的物种补充。这些数据表明，尽管细菌Fur蛋白之间的保守性，H。pylori毛皮含有独特的结构/功能特征，使其与其他物种的毛皮相比具有新颖性。
The Ferric Uptake Regulator (Fur) is a transcriptional regulator that is conserved across a broad number of bacterial species and has been shown to regulate expression of iron uptake and storage genes. Additionally, Fur has been shown to be an important colonization factor of the gastric pathogen Helicobacter pylori. In H. pylori, Fur-dependent regulation appears to be unique in that Fur is able to act as a transcriptional repressor when bound to iron as well as in its iron free (apo) form. To date, apo-regulation has not been identified in any other bacterium. To determine whether Fur from other species has the capacity for apo-regulation, we investigated the ability of Fur from Escherichia coli, Campylobacter jejuni, Desulfovibrio vulgaris Hildenborough, Pseudomonas aeruginosa, and Vibrio cholerae to complement both iron-bound and apo-Fur regulation within the context of an H. pylori fur mutant. We found that while some Fur species (E. coli, C. jejuni and V. cholerae) complemented iron-bound regulation, apo-regulation was unable to be complemented by any of the examined species. These data suggest that despite the conservation among bacterial Fur proteins, H. pylori Fur contains unique structure/function features that make it novel in comparison to Fur from other species.
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影响因子：
3.2
作者：
ERNST, JF;BENNETT, RL;ROTHFIELD, LI
通讯作者：
ROTHFIELD, LI
影响因子：
4.4
作者：
Bender, Kelly S.;Yen, Huei-Che Bill;Wall, Judy D.
通讯作者：
Wall, Judy D.
影响因子：
3.6
作者：
Delany, I;Spohn, G;Scarlato, V
通讯作者：
Scarlato, V
影响因子：
6.4
作者：
Bijlsma, JJE;Lie-A-Ling, M;Kusters, JG
通讯作者：
Kusters, JG
影响因子：
3.2
作者：
Delany, I;Pacheco, ABF;Scarlato, V
通讯作者：
Scarlato, V