Genome-wide census and expression profiling of chicken neuropeptide and prohormone convertase genes.
Genome-wide census and expression profiling of chicken neuropeptide and prohormone convertase genes.
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DOI：
10.1016/j.npep.2009.11.002
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发表时间：
2010-02
期刊：
影响因子：
2.9
通讯作者：
Rodriguez-Zas SL
中科院分区：
文献类型：
--
作者：
Delfino KR;Southey BR;Sweedler JV;Rodriguez-Zas SL
神经肽调节细胞间的信号传导，影响脊椎动物的许多生物学过程，包括发育、生长和繁殖。由激素原转化酶从激素原蛋白质中合成神经肽的复杂过程，再加上经历了广泛的神经肽研究的鸡和哺乳动物物种之间的进化距离，导致只有18种鸡激素原蛋白质的经验证实。为了扩大我们的神经肽和激素原转化酶基因互补的知识，我们进行了详尽的调查，鸡基因组，EST和蛋白质组数据库使用的95个神经肽和7个激素原转化酶基因在其他物种中已知的列表。对EST资源和22个微阵列研究的分析提供了多种条件下基因表达的全面描述。在鸡中发现了5个以前未知的神经肽基因（apelin、可卡因和安非他明调节转录蛋白、胰岛素样5、神经肽S和神经肽B），并确认了62个基因。虽然大多数已知的人类神经肽基因家族存在于鸡中，但有几个基因不存在于鸡中。相反，一些鸡神经肽基因是缺乏哺乳动物物种，包括C-RF酰胺，C型利钠肽1前体，和肾利钠肽。除了一个例外，激素原转化酶是在鸡的基因组中发现的。用于预测激素原裂解的生物信息学模型证实，将激素原蛋白质加工成神经肽在物种之间是相似的。神经肽基因最常在大脑和头部表达，其次是卵巢和小肠。微阵列分析显示，肾上腺髓质素、嗜铬粒蛋白-A、Augurin、neuromedin-U、血小板源性生长因子A和D、脑啡肽原、松弛素-3、prepronociceptin和胰岛素样生长因子I的表达对发育阶段、性别和遗传系的变化最敏感（P值< 0.001）。我们完整的调查和表征有助于了解神经肽基因在鸡，一种重要的生物医学和农业研究的动物。
Neuropeptides regulate cell-cell signaling and influence many biological processes in vertebrates, including development, growth, and reproduction. The complex processing of neuropeptides from prohormone proteins by prohormone convertases, combined with the evolutionary distance between the chicken and mammalian species that have experienced extensive neuropeptide research, has led to the empirical confirmation of only 18 chicken prohormone proteins. To expand our knowledge of the neuropeptide and prohormone convertase gene complement, we performed an exhaustive survey of the chicken genomic, EST, and proteomic databases using a list of 95 neuropeptide and 7 prohormone convertase genes known in other species. Analysis of the EST resources and 22 microarray studies offered a comprehensive portrait of gene expression across multiple conditions. Five neuropeptide genes (apelin, cocaine-and amphetamine-regulated transcript protein, insulin-like 5, neuropeptide S, and neuropeptide B) previously unknown in chicken were identified and 62 genes were confirmed. Although most neuropeptide gene families known in human are present in chicken, there are several gene not present in the chicken. Conversely, several chicken neuropeptide genes are absent from mammalian species, including C-RF amide, c-type natriuretic peptide 1 precursor, and renal natriuretic peptide. The prohormone convertases, with one exception, were found in the chicken genome. Bioinformatic models used to predict prohormone cleavages confirm that the processing of prohormone proteins into neuropeptides is similar between species. Neuropeptide genes are most frequently expressed in the brain and head, followed by the ovary and small intestine. Microarray analyses revealed that the expression of adrenomedullin, chromogranin-A, augurin, neuromedin-U, platelet-derived growth factor A and D, proenkephalin, relaxin-3, prepronociceptin, and insulin-like growth factor I was most susceptible (P-value < 0.001) to changes in developmental stage, gender, and genetic line among other conditions studied. Our complete survey and characterization facilitates understanding of neuropeptides genes in the chicken, an animal of importance to biomedical and agricultural research.
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影响因子：
--
作者：
Aikawa, Shizu;Ishii, Makoto;Sakurai, Takeshi
通讯作者：
Sakurai, Takeshi
影响因子：
4.4
作者：
Cogburn, LA;Wang, X;Simon, J
通讯作者：
Simon, J
DOI：
10.1016/j.ejogrb.2004.07.029
发表时间：
2005-05-01
影响因子：
2.6
作者：
Balat, O;Aksoy, F;Anarat, R
通讯作者：
Anarat, R
影响因子：
1.7
作者：
Florin, S;Suaudeau, C;Costentin, J
通讯作者：
Costentin, J
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y