Nanowarming and ice-free cryopreservation of large sized, intact porcine articular cartilage.
Nanowarming and ice-free cryopreservation of large sized, intact porcine articular cartilage.
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DOI：
10.1038/s42003-023-04577-9
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发表时间：
2023-02-24
影响因子：
5.9
通讯作者：
中科院分区：
文献类型：
--
作者：
成功的器官或组织长期保存将彻底改变生物医学。软骨冷冻保存可以延长关节软骨的保存期，为骨软骨同种异体移植修复软骨提供了广阔的前景。然而，冷冻保存的大尺寸软骨由于其有限的升温速率而不能用传统的对流升温方法成功地升温，从而阻碍了其临床潜力。本研究建立了一种无冰冷冻保存大面积完整关节软骨的方法。我们的方法实现了76.8 °C min-1的加热速率，比对流加热（4.8 °C min-1）高出一个数量级。使用系统的细胞和组织水平的测试，我们证明了我们的方法在保存大型软骨的上级性能。通过磁共振成像和计算机模拟，也观察到了一种深度依赖性的保存方式。最后，我们表明，将纳米颗粒递送到OCA骨侧可能是进一步优化我们方法的可行方向。该研究开创了纳米臂和无冰冷冻保存大型关节软骨的应用，为未来技术发展提供了宝贵的见解，为冷冻保存软骨的临床应用铺平了道路。使用纳米粒子在射频交变磁场中诱导热量以改善加温后性能，将大尺寸关节软骨样品玻璃化并加温。
Successful organ or tissue long-term preservation would revolutionize biomedicine. Cartilage cryopreservation enables prolonged shelf life of articular cartilage, posing the prospect to broaden the implementation of promising osteochondral allograft (OCA) transplantation for cartilage repair. However, cryopreserved large sized cartilage cannot be successfully warmed with the conventional convection warming approach due to its limited warming rate, blocking its clinical potential. Here, we develope a nanowarming and ice-free cryopreservation method for large sized, intact articular cartilage preservation. Our method achieves a heating rate of 76.8 °C min-1, over one order of magnitude higher than convection warming (4.8 °C min-1). Using systematic cell and tissue level tests, we demonstrate the superior performance of our method in preserving large cartilage. A depth-dependent preservation manner is also observed and recapitulated through magnetic resonance imaging and computational modeling. Finally, we show that the delivery of nanoparticles to the OCA bone side could be a feasible direction for further optimization of our method. This study pioneers the application of nanowarming and ice-free cryopreservation for large articular cartilage and provides valuable insights for future technique development, paving the way for clinical applications of cryopreserved cartilage. Large-sized articular cartilage samples are vitrified and warmed using nanoparticles to induce heat in a radiofrequency alternative magnetic field to improve post-warming performance.
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DOI：
10.1053/jars.2002.32839
发表时间：
2002-09-01
影响因子：
4.7
作者：
Hjelle, K;Solheim, E;Brittberg, M
通讯作者：
Brittberg, M
DOI：
10.1016/s0749-8063(97)90124-9
发表时间：
1997-08-01
影响因子：
4.7
作者：
Curl, WW;Krome, J;Poehling, GG
通讯作者：
Poehling, GG
影响因子：
--
作者：
Etheridge, Michael L.;Xu, Yi;Bischof, John C.
通讯作者：
Bischof, John C.
影响因子：
3.7
作者：
Ehrlich, Lili E.;Gao, Zhe;Rabin, Yoed
通讯作者：
Rabin, Yoed
影响因子：
2.7
作者：
Jin B;Kleinhans FW;Mazur P
通讯作者：
Mazur P