Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones.
Comparison of samplers collecting airborne influenza viruses: 1. Primarily impingers and cyclones.
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DOI：
10.1371/journal.pone.0244977
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Goyal SM
中科院分区：
文献类型：
--
作者：
Raynor PC;Adesina A;Aboubakr HA;Yang M;Torremorell M;Goyal SM
研究人员必须能够测量动物农业设施中含病毒颗粒的浓度、大小和传染性，以了解含传染性病毒颗粒在空气中的传播距离，它们可能在人类或动物呼吸道中的存款，以及限制暴露于它们的最有效方法。本研究的目的是评估各种撞击器和旋风气溶胶或生物气溶胶采样器，以确定最适合检测和测量空气中含病毒颗粒浓度的方法。在单独的测试中使用了六个撞击器/旋风空气采样器、一个基于过滤器的采样器和一个级联撞击器，以收集人工产生的MS 2噬菌体和猪流感和禽流感病毒的气溶胶。感染性MS 2大肠杆菌噬菌体的定量使用双琼脂层程序进行。在细胞培养物中滴定流感病毒以确定感染性病毒的量。提取病毒RNA并用于定量真实的时间RT-PCR，以提供所有三种病毒的总病毒浓度。计算回收的病毒量和测得的空气中病毒浓度，并在采样器之间进行比较。毫不奇怪，高流速采样器通常比低流速采样器收集更多的病毒。然而，低流速采样器通常比高流速采样器测量更高且可能更准确的感染性病毒和病毒RNA的空气传播浓度。为了评估现场的空气传播病毒，双采样器方法可能会很好。合适的高流量采样器可以提供低检测限，以确定空气中是否存在任何病毒。如果检测到病毒，适当的低流量采样器可以准确测量空气传播的病毒浓度。
Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effective ways to limit exposures to them. The objective of this study was to evaluate a variety of impinger and cyclone aerosol or bioaerosol samplers to determine approaches most suitable for detecting and measuring concentrations of virus-containing particles in air. Six impinger/cyclone air samplers, a filter-based sampler, and a cascade impactor were used in separate tests to collect artificially generated aerosols of MS2 bacteriophage and swine and avian influenza viruses. Quantification of infectious MS2 coliphage was carried out using a double agar layer procedure. The influenza viruses were titrated in cell cultures to determine quantities of infectious virus. Viral RNA was extracted and used for quantitative real time RT-PCR, to provide total virus concentrations for all three viruses. The amounts of virus recovered and the measured airborne virus concentrations were calculated and compared among the samplers. Not surprisingly, high flow rate samplers generally collected greater quantities of virus than low flow samplers. However, low flow rate samplers generally measured higher, and likely more accurate, airborne concentrations of Infectious virus and viral RNA than high flow samplers. To assess airborne viruses in the field, a two-sampler approach may work well. A suitable high flow sampler may provide low limits of detection to determine if any virus is present in the air. If virus is detected, a suitable lower flow sampler may measure airborne virus concentrations accurately.
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DOI：
10.1080/15428119691014242
发表时间：
1996-12-01
期刊：
AMERICAN INDUSTRIAL HYGIENE ASSOCIATION JOURNAL
影响因子：
--
作者：
Leith, D;Leith, FA;Boundy, MG
通讯作者：
Boundy, MG
影响因子：
11.8
作者：
Arriola CS;Nelson DI;Deliberto TJ;Blanton L;Kniss K;Levine MZ;Trock SC;Finelli L;Jhung MA;H5 Investigation Group
通讯作者：
H5 Investigation Group
影响因子：
3.8
作者：
BENBOUGH, JE
通讯作者：
BENBOUGH, JE
影响因子：
3.1
作者：
IJAZ, MK;KARIM, YG;JOHNSONLUSSENBURG, CM
通讯作者：
JOHNSONLUSSENBURG, CM
影响因子：
5.2
作者：
Agranovski, IE;Safatov, AS;Grinshpun, SA
通讯作者：
Grinshpun, SA