Initial investigation of acoustic droplet vaporization for occlusion in canine kidney.
Initial investigation of acoustic droplet vaporization for occlusion in canine kidney.
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DOI：
10.1016/j.ultrasmedbio.2010.06.020
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发表时间：
2010-10
影响因子：
2.9
通讯作者：
Carson, P. L.
中科院分区：
文献类型：
--
作者：
Zhang, M.;Fabiilli, M. L.;Haworth, K. J.;Fowlkes, J. B.;Kripfgans, O. D.;Roberts, W. W.;Ives, K. A.;Carson, P. L.
关键词：
声学液滴汽化（ADV）显示出空间和时间目标组织闭塞的前景。在这项研究中，通过在肾动脉或节段动脉中产生 ADV 气泡，在手术暴露和经皮的犬肾脏中实现了实质性的组织闭塞。对 15 只犬进行麻醉，其中 10 只接受剖腹手术以取出左肾，5 只未受干扰进行经皮 ADV。微泡是通过封装在血液中的白蛋白或脂质壳中的全氟戊烷液滴发生相变而产生的。 3.5 MHz 单元件治疗传感器与水箱中的成像阵列对齐，可直接进入肾动脉或节段动脉。使用体内彩色血流和频谱多普勒成像来识别目标动脉。占空比为 0.25% 的 1 kHz 脉冲重复频率的音调突发在丸剂通过期间蒸发了液滴。 B 模式成像显示，心内 (IC) 和静脉 (IV) 注射在外化肾脏的选定动脉中反复产生 ADV。在两个案例中，与此同时发生的是通过脉冲波多普勒检测到血流逆转以及波形变窄。在一例中，使用 IC 注射实现了局部皮质闭塞，局部流量减少了 87%。静脉注射的汽化导致回声显着增加，每个液滴剂量的平均半衰期为 8 分钟。通过肾动脉内或静脉注射液滴的经皮汽化产生足以产生一些阴影的气泡，组织路径长达 5.5 cm。
Acoustic droplet vaporization (ADV) shows promise for spatially and temporally targeted tissue occlusion. In this study, substantial tissue occlusion was achieved in operatively exposed and transcutaneous canine kidneys by generating ADV gas bubbles in the renal arteries or segmental arteries. Fifteen canines were anesthetized, among which 10 underwent laparotomy to externalize the left kidney and 5 were undisturbed for transcutaneous ADV. The microbubbles were generated by phase conversion of perfluoropentane droplets encapsulated in albumin or lipid shells in the blood. A 3.5 MHz single-element therapy transducer was aligned with an imaging array in a water tank with direct access to the renal artery or a segmental artery. In vivo color flow and spectral Doppler imaging were used to identify the target arteries. Tone bursts of 1 kHz pulse repetition frequency with 0.25% duty cycle vaporized the droplets during bolus passage. Both intracardiac (IC) and intravenous (IV) injections repeatedly produced ADV in chosen arteries in externalized kidneys, as seen by B-mode imaging. Concurrent with this in two cases was the detection by pulse wave Doppler of blood flow reversal, along with a narrowing of the waveform. Localized cortex occlusion was achieved with 87% regional flow reduction in one case using IC injections. Vaporization from IV injections resulted in a substantial echogenicity increase with an average half-life of 8 minutes per droplet dose. Gas bubbles sufficient to produce some shadowing were generated by transcutaneous vaporization of intra renal artery or IV administered droplets, with a tissue path up to 5.5 cm.
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影响因子：
--
作者：
Kalman, D;Varenhorst, E
通讯作者：
Varenhorst, E
DOI：
10.1109/tuffc.2005.1503996
发表时间：
2005-07-01
影响因子：
3.6
作者：
Kripfgans, OD;Orifici, CM;Fowlkes, JB
通讯作者：
Fowlkes, JB
DOI：
10.1109/tuffc.2002.1009331
发表时间：
2002-06-01
影响因子：
3.6
作者：
Kripfgans, OD;Fowlkes, JB;Carson, PL
通讯作者：
Carson, PL
DOI：
10.3109/10731199409138816
发表时间：
1994-01-01
影响因子：
--
作者：
LEAKAKOS, T;SCHUTT, EG;FLAIM, SF
通讯作者：
FLAIM, SF
DOI：
10.1016/0304-4165(85)90174-6
发表时间：
1985-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
SENIOR, J;CRAWLEY, JCW;GREGORIADIS, G
通讯作者：
GREGORIADIS, G