Subtractive hybridization analysis of gastric diseases-associated Helicobacter pylori identifies peptidyl-prolyl isomerase as a potential marker for gastric cancer.
Subtractive hybridization analysis of gastric diseases-associated Helicobacter pylori identifies peptidyl-prolyl isomerase as a potential marker for gastric cancer.
复制标题
对胃病相关幽门螺杆菌的消减杂交分析确定肽基脯氨酰异构酶是胃癌的潜在标志物。
DOI：
10.1111/j.1574-6968.2011.02296.x
复制
发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Yuan Yuan
中科院分区：
文献类型：
--
作者：
Yue-hua Gong;Moye Chen;Ying Xu;Nannan Dong;Zhikun Sang;Jun Liu;Yuan Yuan
幽门螺杆菌是一种微嗜氧革兰氏阴性细菌，可导致慢性胃炎、消化性溃疡和胃癌。某些菌株中存在的基因可能决定特定菌株的特征，如疾病关联性和抗药性。为了了解胃病的发病机制，寻找幽门螺杆菌相关疾病的分子标志物，为幽门螺杆菌相关疾病的靶向治疗提供线索，采用抑制性消减杂交技术构建了胃癌相关幽门螺杆菌和浅表性胃炎相关幽门螺杆菌的消减DNA文库。通过斑点杂交、DNA测序和基于聚合酶链式反应的筛选来鉴定胃癌特异性基因的存在。与浅表性胃炎相比，胃癌组织中发现了12个胃癌特异性高拷贝基因和9个低拷贝基因。通过对幽门螺杆菌分离株的PCR分析，证实了这些基因的存在。值得注意的是，在22株与胃癌相关的幽门螺杆菌菌株中，有11株(50%)的PPIase呈阳性。相比之下，浅表性胃炎的幽门螺杆菌菌株中有24%呈阳性结果。鉴于PPIase在细胞生长、凋亡和致癌转化中的潜在作用，我们的结果表明PPIase可能是一种新的标记物和潜在的胃癌治疗靶点。
Helicobacter pylori, a microaerophilic Gram-negative bacterium, is known to cause chronic gastritis, peptic ulcer and gastric cancer. Genes that are present in certain isolates may determine strain-specific traits such as disease association and drug resistance. In order to understand the pathogenic mechanisms of gastric diseases, identify molecular markers of the diseases associated with H. pylori strains and provide clues for target treatment of H. pylori-related diseases, a subtracted DNA library was constructed from a gastric cancer-associated H. pylori strain and a superficial gastritis-associated H. pylori strain by suppression subtractive hybridization. The presence of gastric cancer-specific genes was identified by dot blot hybridization, DNA sequencing and PCR-based screening. Twelve gastric cancer-specific high-copy genes and nine low-copy genes were found in gastric cancer compared with the superficial gastritis strain. These genes were confirmed by PCR analysis of H. pylori isolates. Notably, peptidyl-prolyl cis-trans isomerase (PPIase) was detected positively in 11 out of 22 (50%) gastric cancer-associated H. pylori strains. In contrast, <24% of the H. pylori strains from superficial gastritis showed positive results. Given the potential role of PPIases in cell growth, apoptosis and oncogenic transformation, our results suggest that PPIase may represent a novel marker and potential therapeutic target for gastric cancer.
登录
查看更多内容
DOI：
10.1002/bies.20768
发表时间：
2008-06
期刊：
BioEssays : news and reviews in molecular, cellular and developmental biology
影响因子：
--
作者：
H. Mimuro;D. Berg;C. Sasakawa
通讯作者：
H. Mimuro;D. Berg;C. Sasakawa
DOI：
10.1186/1756-9966-27-38
发表时间：
2008-09-15
影响因子：
11.3
作者：
Campioni, Mara;Ambrogi, Vincenzo;Pompeo, Eugenio;Citro, Gennaro;Castelli, Mauro;Spugnini, Enrico P.;Gatti, Antonio;Cardelli, Pierluigi;Lorenzon, Laura;Baldi, Alfonso;Mineo, Tommaso C.
通讯作者：
Mineo, Tommaso C.
DOI：
10.1073/pnas.90.12.5791
发表时间：
1993-06-15
影响因子：
11.1
作者：
COVACCI, A;CENSINI, S;RAPPUOLI, R
通讯作者：
RAPPUOLI, R
影响因子：
2.4
作者：
O. Olivares-Fuster;C. Arias
通讯作者：
O. Olivares-Fuster;C. Arias
影响因子：
2.1
作者：
Salaün, L;Audibert, C;Picard, B
通讯作者：
Picard, B