Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors.
Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors.
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DOI：
10.1007/s00253-011-3408-y
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发表时间：
2011-08
影响因子：
5
通讯作者：
Lok, Si
中科院分区：
文献类型：
--
作者：
Zhang, Tong;Ye, Lin;Tong, Amy Hin Yan;Shao, Ming-Fei;Lok, Si
关键词：
采用双脱氧测序和454高通量测序技术，对6个城市污水处理厂氨氧化古菌（AOA）和氨氧化细菌（AOB）的氨单加氧酶（amoA）基因和16 S rRNA基因进行了分析。结果表明，不同污水处理厂的AOB amoA基因存在较大差异，而16 SrRNA基因相对保守。基于观察到的amoA和16 S rRNA基因的复杂性，大多数AOB可以归属于亚硝化单胞菌属，其中亚硝化单胞菌属，寡养亚硝化单胞菌属，海洋亚硝化单胞菌属和亚硝化单胞菌属是四个最主要的物种。从AOA amoA基因的序列来看，本研究中观察到的大多数AOA属于CGI.1b组，即，土壤血统AOB amoA和16 S rRNA基因分别通过定量PCR和454高通量焦磷酸测序进行定量。虽然两种方法的结果显示出一些不一致，但它们都表明活性污泥中AOB的丰度很低。本文的在线版本（doi：10.1007/s 00253 -011-3408-y）包含补充材料，可供授权用户使用。
In this study, dideoxy sequencing and 454 high-throughput sequencing were used to analyze diversities of the ammonia monooxygenase (amoA) genes and the 16S rRNA genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in six municipal wastewater treatment plants. The results showed that AOB amoA genes were quite diverse in different wastewater treatment plants while the 16S rRNA genes were relatively conserved. Based on the observed complexity of amoA and 16S rRNA genes, most of the AOB can be assigned to the Nitrosomonas genus, with Nitrosomonas ureae, Nitrosomonas oligotropha, Nitrosomonas marina, and Nitrosomonas aestuarii being the four most dominant species. From the sequences of the AOA amoA genes, most AOA observed in this study belong to the CGI.1b group, i.e., the soil lineage. The AOB amoA and 16S rRNA genes were quantified by quantitative PCR and 454 high-throughput pyrosequencing, respectively. Although the results from the two approaches show some disconcordance, they both indicated that the abundance of AOB in activated sludge was very low. The online version of this article (doi:10.1007/s00253-011-3408-y) contains supplementary material, which is available to authorized users.
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