Toward a Transportable Cell Culture Platform for Evaluating Radiotherapy Dose Modifying Factors.
Toward a Transportable Cell Culture Platform for Evaluating Radiotherapy Dose Modifying Factors.
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DOI：
10.3390/ijms242115953
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发表时间：
2023-11-03
影响因子：
5.6
通讯作者：
Tambasco M
中科院分区：
文献类型：
--
作者：
Carlson N;House CD;Tambasco M
目前在放射治疗中验证剂量输送和优化新放射治疗技术的工具并未考虑重要的剂量修改因素（DMF），例如细胞修复能力的变化、肿瘤氧合作用、超高剂量率和所使用的电离辐射类型。这些因素在肿瘤控制和正常组织并发症中发挥着至关重要的作用。为了满足这一需求，我们探索了开发可运输细胞培养平台（TCCP）来评估电离辐射的相对生物有效性（RBE）的可行性。我们在常氧和低氧环境下，在室温下、在补充培养基的明胶中添加一定浓度的胎牛血清 (FBS) 的情况下，在几天内测量了 MDA-MB-231 细胞的细胞恢复、克隆形成活力和代谢活力。此外，我们还测量了细胞的克隆形成活力，以表征室温下 TCCP 的持续时间如何影响其在高达 16 Gy 剂量下的放射敏感性。我们发现，在缺氧 (pO2) 的条件下，在添加有 50% FBS 的明胶培养基中室温保存三天后，MDA-MB-231 细胞成功恢复，同时通过 ATP 发光和集落形成测量的代谢和克隆形成活力分别为 和 %。此外，在常氧和缺氧条件下照射 TCCP 产生的克隆形成氧增强比 (OER) 和代谢 OER我们的结果表明，TCCP 可用于评估 DMF 的 RBE，并为评估放射治疗应用中的 DMF 提供可行的平台。
The current tools for validating dose delivery and optimizing new radiotherapy technologies in radiation therapy do not account for important dose modifying factors (DMFs), such as variations in cellular repair capability, tumor oxygenation, ultra-high dose rates and the type of ionizing radiation used. These factors play a crucial role in tumor control and normal tissue complications. To address this need, we explored the feasibility of developing a transportable cell culture platform (TCCP) to assess the relative biological effectiveness (RBE) of ionizing radiation. We measured cell recovery, clonogenic viability and metabolic viability of MDA-MB-231 cells over several days at room temperature in a range of concentrations of fetal bovine serum (FBS) in medium-supplemented gelatin, under both normoxic and hypoxic oxygen environments. Additionally, we measured the clonogenic viability of the cells to characterize how the duration of the TCCP at room temperature affected their radiosensitivity at doses up to 16 Gy. We found that of MDA-MB-231 cells were successfully recovered after being kept at room temperature for three days in 50% FBS in medium-supplemented gelatin at hypoxia ( pO2, while metabolic and clonogenic viabilities as measured by ATP luminescence and colony formation were found to be and %, respectively. Additionally, irradiating a TCCP under normoxic and hypoxic conditions yielded a clonogenic oxygen enhancement ratio (OER) of and a metabolic OER of . Our results demonstrate that the TCCP can be used to assess the RBE of a DMF and provides a feasible platform for assessing DMFs in radiation therapy applications.
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DOI：
10.32907/ro-117-6669
发表时间：
2020
期刊：
Research outreach : the outreach quarterly connecting science with society
影响因子：
--
作者：
Petersson K;Collaborators
通讯作者：
Collaborators
影响因子：
3.6
作者：
Baskar R;Lee KA;Yeo R;Yeoh KW
通讯作者：
Yeoh KW
影响因子：
45.3
作者：
Fyles, A;Milosevic, M;Hill, RP
通讯作者：
Hill, RP
影响因子：
2.3
作者：
Hanna, Jacob;Hubel, Allison
通讯作者：
Hubel, Allison
影响因子：
2.2
作者：
Lartigau, E;Randrianarivelo, H;Guichard, M
通讯作者：
Guichard, M