Divergent Regulation of CBF Regulon on Cold Tolerance and Plant Phenotype in Cassava Overexpressing Arabidopsis CBF3 Gene.
Divergent Regulation of CBF Regulon on Cold Tolerance and Plant Phenotype in Cassava Overexpressing Arabidopsis CBF3 Gene.
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CBF调节子对过表达拟南芥CBF3基因的木薯耐冷性和植物表型的发散调控
DOI：
10.3389/fpls.2016.01866
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zhang P
中科院分区：
文献类型：
--
作者：
An D;Ma Q;Yan W;Zhou W;Liu G;Zhang P
木薯是一种对低温胁迫敏感的热带植物。为了理解CBF冷反应途径，在温带植物中公认的调节机制，在木薯中，研究了拟南芥CBF3基因的过表达。该基因使木薯对寒冷和干旱胁迫的耐受性增加，但与植物生长迟缓、叶片卷曲、贮藏根产量降低和花青素苷积累减少有关，其转录本丰度依赖性方式。生理分析表明，转基因木薯增加脯氨酸积累，减少丙二醛的产生，和电解质渗漏在低温胁迫下。这些转基因株系在干旱条件下也表现出较高的相对含水量。在野生型和转基因植物中，部分CBF靶向基因的表达响应于冷显示时间和空间变化：在叶片中高度诱导，在顶芽中较少改变。此外，花青素的积累被抑制下调基因的表达参与其生物合成和CBF3和内源性转录因子之间的相互作用。因此，异源CBF3调节应激相关基因的表达，并在应激条件下进行一系列生理调节，显示出与木薯CBF不同的CBF调节子调节模式。
Cassava is a tropical origin plant that is sensitive to chilling stress. In order to understand the CBF cold response pathway, a well-recognized regulatory mechanism in temperate plants, in cassava, overexpression of an Arabidopsis CBF3 gene is studied. This gene renders cassava increasingly tolerant to cold and drought stresses but is associated with retarded plant growth, leaf curling, reduced storage root yield, and reduced anthocyanin accumulation in a transcript abundance-dependent manner. Physiological analysis revealed that the transgenic cassava increased proline accumulation, reduced malondialdehyde production, and electrolyte leakage under cold stress. These transgenic lines also showed high relative water content when faced with drought. The expression of partial CBF-targeted genes in response to cold displayed temporal and spatial variations in the wild-type and transgenic plants: highly inducible in leaves and less altered in apical buds. In addition, anthocyanin accumulation was inhibited by downregulating the expression of genes involved in its biosynthesis and by interplaying between the CBF3 and the endogenous transcription factors. Thus, the heterologous CBF3 modulates the expression of stress-related genes and carries out a series of physiological adjustments under stressful conditions, showing a varied regulation pattern of CBF regulon from that of cassava CBFs.
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影响因子：
4.4
作者：
An D;Yang J;Zhang P
通讯作者：
Zhang P
影响因子：
7.4
作者：
Jaglo, KR;Kleff, S;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
DOI：
10.1073/pnas.191389398
发表时间：
2001-09-25
影响因子：
11.1
作者：
Gaxiola, RA;Li, JS;Fink, GR
通讯作者：
Fink, GR
影响因子：
46.9
作者：
Bredeson, Jessen V.;Lyons, Jessica B.;Rokhsar, Daniel S.
通讯作者：
Rokhsar, Daniel S.