Comparative and functional analysis of intron-mediated enhancement signals reveals conserved features among plants.
Comparative and functional analysis of intron-mediated enhancement signals reveals conserved features among plants.
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DOI：
10.1093/nar/gkr043
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发表时间：
2011-07
影响因子：
14.9
通讯作者：
Korf I
中科院分区：
文献类型：
--
作者：
Parra G;Bradnam K;Rose AB;Korf I
在包括植物、动物和真菌在内的广泛生物体中的内含子能够增加它们所包含的基因的表达。内含子介导的增强（IME）的过程在拟南芥中被最彻底地研究，其中已经显示增强内含子通常位于启动子附近并且在组成上不同于下游内含子。在这项研究中，我们进行了全面的比较分析几个测序植物基因组。我们发现增强序列在多细胞植物中是保守的，但在藻类中不存在或无法识别。IME信号优先位于第一内含子的5′-末端，但似乎也富集在转录起始位点附近的5′-UTR和编码区。增强型内含子在广泛组织中高度表达的基因中最为显著。通过对A. thaliana，我们表明IME信号可以插入或删除内含子，以增加或减少基因表达。虽然我们还不知道IME的具体机制，但预测的信号似乎既有功能又高度保守。
Introns in a wide range of organisms including plants, animals and fungi are able to increase the expression of the gene that they are contained in. This process of intron-mediated enhancement (IME) is most thoroughly studied in Arabidopsis thaliana, where it has been shown that enhancing introns are typically located near the promoter and are compositionally distinct from downstream introns. In this study, we perform a comprehensive comparative analysis of several sequenced plant genomes. We find that enhancing sequences are conserved in the multi-cellular plants but are either absent or unrecognizable in algae. IME signals are preferentially located towards the 5′-end of first introns but also appear to be enriched in 5′-UTRs and coding regions near the transcription start site. Enhancing introns are found most prominently in genes that are highly expressed in a wide range of tissues. Through site-directed mutagenesis in A. thaliana, we show that IME signals can be inserted or removed from introns to increase or decrease gene expression. Although we do not yet know the specific mechanism of IME, the predicted signals appear to be both functional and highly conserved.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
14.9
作者：
Nogales-Cadenas R;Carmona-Saez P;Vazquez M;Vicente C;Yang X;Tirado F;Carazo JM;Pascual-Montano A
通讯作者：
Pascual-Montano A
影响因子：
14.9
作者：
Touchon, M;Arneodo, A;Thermes, C
通讯作者：
Thermes, C
影响因子：
10.5
作者：
CALLIS, J;FROMM, M;WALBOT, V
通讯作者：
WALBOT, V
影响因子：
11.4
作者：
Jeffares, Daniel C.;Penkett, Christopher J.;Baehler, Juerg
通讯作者：
Baehler, Juerg