Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells.
Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells.
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DOI：
10.1002/jbm.a.32696
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发表时间：
2010-08
影响因子：
4.9
通讯作者：
Yaszemski, Michael J.
中科院分区：
文献类型：
--
作者：
Hesse, Eric;Hefferan, Theresa E.;Tarara, James E.;Haasper, Carl;Meller, Rupert;Krettek, Christian;Lu, Lichun;Yaszemski, Michael J.
关键词：
水凝胶在再生医学中具有潜在的用途，包括药物和生长因子递送，作为骨修复的单一支架或作为另一种生物材料的孔的填充物，其中宿主间充质祖细胞可以迁移并分化成基质产生的成骨细胞。I型胶原蛋白特别令人感兴趣，因为它是一种非常重要且丰富的天然基质成分。本研究的目的是探讨大鼠骨髓基质细胞（rBMSCs）是否能够粘附，生存，增殖和迁移的胶原I型水凝胶，以及他们是否可以通过成骨细胞的命运。从大鼠股骨获得rBMSC并将其铺在I型胶原水凝胶上。在第7、14和21天收获之前，将水凝胶荧光标记、冷冻切割并通过基于荧光的和激光扫描共聚焦显微镜分析以确定细胞增殖、迁移和活力。碱性磷酸酶活性测定成骨分化。I型胶原水凝胶允许rBMSC附着于水凝胶，它们的增殖和向凝胶内部的迁移。培养两周后，碱性磷酸酶活性增加，表明rBMSCs开始分化为成骨细胞。因此，这项研究表明，I型胶原蛋白水凝胶可用于肌肉骨骼再生疗法。
Hydrogels are potentially useful for many purposes in regenerative medicine including drug and growth factor delivery, as single scaffold for bone repair or as a filler of pores of another biomaterial in which host mesenchymal progenitor cells can migrate in and differentiate into matrix-producing osteoblasts. Collagen type I is of special interest as it is a very important and abundant natural matrix component. The purpose of this study was to investigate whether rat bone marrow stromal cells (rBMSCs) are able to adhere to, to survive, to proliferate and to migrate in collagen type I hydrogels and whether they can adopt an osteoblastic fate. rBMSCs were obtained from rat femora and plated on collagen type I hydrogels. Prior to harvest by day 7, 14, and 21, hydrogels were fluorescently labeled, cryo-cut and analyzed by fluorescent-based and laser scanning confocal microscopy to determine cell proliferation, migration, and viability. Osteogenic differentiation was determined by alkaline phosphatase activity. Collagen type I hydrogels allowed the attachment of rBMSCs to the hydrogel, their proliferation, and migration towards the inner part of the gel. rBMSCs started to differentiate into osteoblasts as determined by an increase in alkaline phosphatase activity after two weeks in culture. This study therefore suggests that collagen type I hydrogels could be useful for musculoskeletal regenerative therapies.
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影响因子：
2.6
作者：
Mankani, Mahesh H.;Kuznetsov, Sergei A.;Robey, Pamela Gehron
通讯作者：
Robey, Pamela Gehron
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
DOI：
10.2106/00004623-200205000-00003
发表时间：
2002-05-01
影响因子：
5.3
作者：
Ahlmann, E;Patzakis, M;Holtom, P
通讯作者：
Holtom, P
DOI：
10.1007/s10856-006-0615-7
发表时间：
2006-12-01
影响因子：
3.7
作者：
Cui, F. Z.;Tian, W. M.;Lee, I. -S.
通讯作者：
Lee, I. -S.
影响因子：
4.9
作者：
Betz, Martha W.;Modi, Parth C.;Fisher, John P.
通讯作者：
Fisher, John P.