An integrative analysis of the transcriptome and proteome of the pulp of a spontaneous late-ripening sweet orange mutant and its wild type improves our understanding of fruit ripening in citrus.
An integrative analysis of the transcriptome and proteome of the pulp of a spontaneous late-ripening sweet orange mutant and its wild type improves our understanding of fruit ripening in citrus.
复制标题
DOI：
10.1093/jxb/eru044
复制
发表时间：
2014-04
影响因子：
6.9
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Wu J;Xu Z;Zhang Y;Chai L;Yi H;Deng X
阿坝、乙烯、蔗糖及其相关基因和通路参与了柑橘果实的成熟，阿坝可能在果实成熟过程中起着核心作用。果实成熟是一个复杂的、遗传程序化的过程，其与叶绿体分化成色素体一起发生，并且涉及果实的感官特性的变化。本研究采用转录组和蛋白质组相结合的方法，对自然晚熟突变体（'丰湾'橙子，Citrus sinensis L.）Osbeck）及其野生型（“奉节72- 1”）。在转录水平上，628个基因在突变体和野生型之间显示出2倍或更多的表达差异，如通过RNA测序方法检测到的。在蛋白质水平上，130种蛋白质的相对丰度相差1.5倍或更多，如iTRAQ（相对和绝对定量的同量异位素标签）分析所示。转录组和蛋白质组数据的比较揭示了橙子果实成熟过程中代谢调控的某些方面。首先，发现大量差异基因属于植物激素途径和细胞壁相关代谢。其次，我们注意到成熟相关转录物和糖代谢产物之间的相关性，这表明这些代谢途径在果实成熟过程中的重要性。第三，许多基因在转录和蛋白质水平之间表现出不一致性，这是转录后事件的指示。这些结果揭示了柑橘成熟过程中的多个成熟相关事件，并为柑橘成熟调控网络的分子机制提供了新的见解。
ABA, ethylene, sucrose, and their related genes and pathways are involved in fruit ripening of citrus, and ABA may play a central role during the ripening process. Fruit ripening is a complex, genetically programmed process that occurs in conjunction with the differentiation of chloroplasts into chromoplasts and involves changes to the organoleptic properties of the fruit. In this study, an integrative analysis of the transcriptome and proteome was performed to identify important regulators and pathways involved in fruit ripening in a spontaneous late-ripening mutant (‘Fengwan’ orange, Citrus sinensis L. Osbeck) and its wild type (‘Fengjie 72-1’). At the transcript level, 628 genes showed a 2-fold or more expression difference between the mutant and wild type as detected by an RNA sequencing approach. At the protein level, 130 proteins differed by 1.5-fold or more in their relative abundance, as indicated by iTRAQ (isobaric tags for relative and absolute quantitation) analysis. A comparison of the transcriptome and proteome data revealed some aspects of the regulation of metabolism during orange fruit ripening. First, a large number of differential genes were found to belong to the plant hormone pathways and cell-wall-related metabolism. Secondly, we noted a correlation between ripening-associated transcripts and sugar metabolites, which suggests the importance of these metabolic pathways during fruit ripening. Thirdly, a number of genes showed inconsistency between the transcript and protein level, which is indicative of post-transcriptional events. These results reveal multiple ripening-associated events during citrus ripening and provide new insights into the molecular mechanisms underlying citrus ripening regulatory networks.
登录
查看更多内容
影响因子：
6.9
作者：
Katz E;Boo KH;Kim HY;Eigenheer RA;Phinney BS;Shulaev V;Negre-Zakharov F;Sadka A;Blumwald E
通讯作者：
Blumwald E
影响因子：
4.4
作者：
Gan, Chee Sian;Chong, Poh Kuan;Wright, Phillip C.
通讯作者：
Wright, Phillip C.
影响因子：
3
作者：
Ji, Kai;Chen, Pei;Leng, Ping
通讯作者：
Leng, Ping
2C型蛋白磷酸酶ABI1是草莓果实成熟的负调节因子
影响因子：
6.9
作者：
Jia HF;Lu D;Sun JH;Li CL;Xing Y;Qin L;Shen YY
通讯作者：
Shen YY
影响因子：
6.9
作者：
Johnston JW;Gunaseelan K;Pidakala P;Wang M;Schaffer RJ
通讯作者：
Schaffer RJ