Type 2C protein phosphatase ABI1 is a negative regulator of strawberry fruit ripening.
Type 2C protein phosphatase ABI1 is a negative regulator of strawberry fruit ripening.
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2C型蛋白磷酸酶ABI1是草莓果实成熟的负调节因子
DOI：
10.1093/jxb/ert028
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发表时间：
2013-04
影响因子：
6.9
通讯作者：
Shen YY
中科院分区：
文献类型：
--
作者：
Jia HF;Lu D;Sun JH;Li CL;Xing Y;Qin L;Shen YY
虽然在了解脱落酸(ABA)在果实成熟过程中的作用方面已经取得了很大的进展，但ABA信号通路中的许多成分仍有待阐明。在此，我们分离并鉴定了一个与拟南芥基因ABI1同源的草莓基因，命名为FaABI1。1641bpcDNA包括一个完整的开放阅读框，编码546个氨基酸组成的蛋白，同源性分析确定了其保守结构域。实时定量聚合酶链式反应、半定量逆转录聚合酶链式反应和Northern印迹杂交分析表明，在草莓果实发育过程中，FaABI1基因的表达水平迅速下降，表明FaABI1编码的丝氨酸/苏氨酸蛋白磷酸酶PP2C1可能作为负调控基因参与果实成熟过程。烟草骚动病毒诱导的基因沉默和PBI121载体介导的过表达结果表明，草莓果实脱脂过程中FaABI1基因表达水平的下调和上调分别起到促进和抑制成熟的作用。此外，FaABI1表达的改变可以差异地调控一组与ABA反应和成熟相关的基因的转录，包括ABI3、ABI4、ABI5、SnRK2、ABRE1、CHS、PG1、PL、CHI、F3H、DFR、ANS和UFGT。综上所述，这些数据为ABA在调控草莓果实成熟过程中发挥重要作用提供了新的证据，而2C蛋白磷酸酶ABI1在这一过程中起负调控作用。最后，讨论了草莓果实成熟过程中ABA感知和信号转导的可能核心机制。
Although a great deal of progress has been made toward understanding the role of abscisic acid (ABA) in fruit ripening, many components in the ABA signalling pathway remain to be elucidated. Here, a strawberry gene homologous to the Arabidopsis gene ABI1, named FaABI1, was isolated and characterized. The 1641bp cDNA includes an intact open reading frame that encodes a deduced protein of 546 amino acids, in which putative conserved domains were determined by homology analysis. Transcriptional analysis showed that the levels of FaABI1 mRNA expression declined rapidly during strawberry fruit development as evidenced by real-time PCR, semi-quantitative reverse transcription–PCR, and northern blotting analyses, suggesting that the Ser/Thr protein phosphatase PP2C1 encoded by FaABI1 may be involved in fruit ripening as a negative regulator. The results of Tobacco rattle virus-induced gene silencing and PBI121 vector-mediated overexpression suggested that the down- and up-regulation of FaABI1 mRNA expression levels in degreening strawberry fruit could promote and inhibit ripening, respectively. Furthermore, alteration of FaABI1 expression could differentially regulate the transcripts of a set of both ABA-responsive and ripening-related genes, including ABI3, ABI4, ABI5, SnRK2, ABRE1, CHS, PG1, PL, CHI, F3H, DFR, ANS, and UFGT. Taken together, the data provide new evidence for an important role for ABA in regulating strawberry fruit ripening in the processes of which the type 2C protein phosphatase ABI1 serves as a negative regulator. Finally, a possible core mechanism underlying ABA perception and signalling transduction in strawberry fruit ripening is discussed.
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脱甲酸受体的激素信号传导的栅极锁锁机制。
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
56.9
作者：
MEYER, K;LEUBE, MP;GRILL, E
通讯作者：
GRILL, E
影响因子：
64.8
作者：
Miyazono, Ken-ichi;Miyakawa, Takuya;Tanokura, Masaru
通讯作者：
Tanokura, Masaru
影响因子：
56.9
作者：
LEUNG, J;BOUVIERDURAND, M;GIRAUDAT, J
通讯作者：
GIRAUDAT, J
DOI：
10.1111/j.1432-1033.1992.tb17364.x
发表时间：
1992-11-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
MACKINTOSH, RW;DAVIES, SP;HARDIE, DG
通讯作者：
HARDIE, DG