A Hybrid Titanium-Softmaterial, High-Strength, Transparent Cranial Window for Transcranial Injection and Neuroimaging.
A Hybrid Titanium-Softmaterial, High-Strength, Transparent Cranial Window for Transcranial Injection and Neuroimaging.
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DOI：
10.3390/bios12020129
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发表时间：
2022-02-18
期刊：
影响因子：
--
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Yang N;Liu F;Zhang X;Chen C;Xia Z;Fu S;Wang J;Xu J;Cui S;Zhang Y;Yi M;Wan Y;Li Q;Xu S
透明且可穿透的颅窗对于神经成像、经颅注射和全面了解皮质功能至关重要。对于这些应用，由玻璃盖盖，聚二甲基硅氧烷（PDMS），聚甲基丙烯酸甲酯，晶体和硅水凝胶制成的颅窗提供了显着的便利。然而，目前缺乏具有临床应用潜力的高强度、高透明度、可穿透性的颅窗。我们设计了高强度混合钛- pdms （Ti-PDMS）颅窗，它允许大的透明区域进行体内双光子成像，并为经颅注射提供了一个软窗。利用激光扫描和3D打印技术将混合颅窗与不同的颅骨形态进行匹配。多循环脱气浇注工艺确保了PDMS与Ti框架的良好结合。Ti-PDMS颅窗具有与人类颅骨相匹配的高断裂强度，优异的透光率高达94.4%，折射率接近生物组织。Ti-PDMS颅窗在小鼠体内植入21周后表现出良好的生物相容性。染料注射表明，PDMS窗口具有“自密封”功能，可以防止液体泄漏。脑组织的双光子成像可以实现高达450 μ m的z深度。作为一种新型的脑机接口，Ti-PDMS装置为体内给药、光学实验、超声治疗和电生理记录提供了另一种选择。
A transparent and penetrable cranial window is essential for neuroimaging, transcranial injection and comprehensive understanding of cortical functions. For these applications, cranial windows made from glass coverslip, polydimethylsiloxane (PDMS), polymethylmethacrylate, crystal and silicone hydrogel have offered remarkable convenience. However, there is a lack of high-strength, high-transparency, penetrable cranial window with clinical application potential. We engineer high-strength hybrid Titanium-PDMS (Ti-PDMS) cranial windows, which allow large transparent area for in vivo two-photon imaging, and provide a soft window for transcranial injection. Laser scanning and 3D printing techniques are used to match the hybrid cranial window to different skull morphology. A multi-cycle degassing pouring process ensures a good combination of PDMS and Ti frame. Ti-PDMS cranial windows have a high fracture strength matching human skull bone, excellent light transmittance up to 94.4%, and refractive index close to biological tissue. Ti-PDMS cranial windows show excellent bio-compatibility during 21-week implantation in mice. Dye injection shows that the PDMS window has a “self-sealing” to keep liquid from leaking out. Two-photon imaging for brain tissues could be achieved up to 450 µm in z-depth. As a novel brain-computer-interface, this Ti-PDMS device offers an alternative choice for in vivo drug delivery, optical experiments, ultrasonic treatment and electrophysiology recording.
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影响因子：
14.8
作者：
Holtmaat, Anthony;Bonhoeffer, Tobias;Chow, David K.;Chuckowree, Jyoti;De Paola, Vincenzo;Hofer, Sonja B.;Huebener, Mark;Keck, Tara;Knott, Graham;Lee, Wei-Chung A.;Mostany, Ricardo;Mrsic-Flogel, Tom D.;Nedivi, Elly;Portera-Cailliau, Carlos;Svoboda, Karel;Trachtenberg, Joshua T.;Wilbrecht, Linda
通讯作者：
Wilbrecht, Linda
微流体应用的PDMS键合技术：评论。
DOI：
10.3390/bios11080292
发表时间：
2021-08-23
期刊：
Biosensors
影响因子：
--
作者：
Borók A;Laboda K;Bonyár A
通讯作者：
Bonyár A
未损伤腓肠神经中 Ca(v)3.2 T 型钙通道的积累导致神经损伤大鼠的神经病理性疼痛
影响因子：
4.8
作者：
Chen W;Chi YN;Kang XJ;Liu QY;Zhang HL;Li ZH;Zhao ZF;Yang Y;Su L;Cai J;Liao FF;Yi M;Wan Y;Liu FY
通讯作者：
Liu FY
影响因子：
5.4
作者：
Gernert M;Feja M
通讯作者：
Feja M
影响因子：
3
作者：
Cramer SW;Carter RE;Aronson JD;Kodandaramaiah SB;Ebner TJ;Chen CC
通讯作者：
Chen CC