Optimizing Production of Two Potential Probiotic Lactobacilli Strains Isolated from Piglet Feces as Feed Additives for Weaned Piglets.
Optimizing Production of Two Potential Probiotic Lactobacilli Strains Isolated from Piglet Feces as Feed Additives for Weaned Piglets.
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DOI：
10.5713/ajas.14.0780
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Chen MJ
中科院分区：
文献类型：
--
作者：
Chiang ML;Chen HC;Chen KN;Lin YC;Lin YT;Chen MJ
两株益生菌，约氏乳杆菌x-1d-2和粘膜乳杆菌x-4 w-1，最初从仔猪粪便中分离，已被证明具有抗菌活性，抗生素抗性和白细胞介素-6诱导能力在RAW 267.4巨噬细胞在我们以前的研究。这些特点使L. johnsonii x-1d-2和L. mucosae x-4 w-1是应用于饲料益生菌的良好候选者。本研究以豆粕、糖蜜和醋酸钠为主要原料，对乳酸菌的生长培养基进行了优化。johnsonii x-1d-2和L.粘膜x-4 w-1。然后将这两种菌株冷冻干燥并混合到基础日粮中饲喂断奶仔猪。L. johnsonii x-1d-2和L.本试验研究了X-4 W-1型肠粘膜活菌制剂对断奶仔猪生产性能和粪便微生物菌群的影响。结果表明，L. johnsonii x-1d-2和L. mucosae x-4 w-1在由5.5%（wt/vol）豆粕、1.0%（wt/vol）糖蜜和1.0%（wt/vol）乙酸钠组成的最佳培养基中分别达到8.90和9.30 log CFU/mL的最大值。培养基成本比市售的de Man、Rogosa和Sharpe培养基低96%。在进一步的饲养研究中，饲喂补充有冻干益生菌培养物的基础饮食的断奶仔猪表现出比饲喂基础饮食的断奶仔猪更高（p<0.05）的体重增加、采食量和增重/饲料比。益生菌喂养也增加了乳酸杆菌的数量，减少了E。断奶仔猪粪便中的大肠杆菌。本研究表明L. johnsonii x-1d-2和L. Mucosae X-4 W-1具有作为饲料添加剂用于养猪业的高潜力。
Two probiotic strains, Lactobacillus johnsonii x-1d-2 and Lactobacillus mucosae x-4w-1, originally isolated from piglet feces, have been demonstrated to possess antimicrobial activities, antibiotic resistances and interleukin-6 induction ability in RAW 267.4 macrophages in our previous study. These characteristics make L. johnsonii x-1d-2 and L. mucosae x-4w-1 good candidates for application in feed probiotics. In this study, soybeal meal, molasses and sodium acetate were selected to optimize the growth medium for cultivation of L. johnsonii x-1d-2 and L. mucosae x-4w-1. These two strains were then freeze-dried and mixed into the basal diet to feed the weaned piglets. The effects of L. johnsonii x-1d-2 and L. mucosae x-4w-1 on the growth performance and fecal microflora of weaned piglets were investigated. The results showed that the bacterial numbers of L. johnsonii x-1d-2 and L. mucosae x-4w-1 reached a maximum of 8.90 and 9.30 log CFU/mL, respectively, when growing in optimal medium consisting of 5.5% (wt/vol) soybean meal, 1.0% (wt/vol) molasses and 1.0% (wt/vol) sodium acetate. The medium cost was 96% lower than the commercial de Man, Rogosa and Sharpe medium. In a further feeding study, the weaned piglets fed basal diet supplemented with freeze-dried probiotic cultures exhibited higher (p<0.05) body weight gain, feed intake, and gain/feed ratio than weaned piglets fed basal diet. Probiotic feeding also increased the numbers of lactobacilli and decreased the numbers of E. coli in the feces of weaned piglets. This study demonstrates that L. johnsonii x-1d-2 and L. mucosae x-4w-1 have high potential to be used as feed additives in the pig industry.
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影响因子：
5.9
作者：
Van Tassell ML;Miller MJ
通讯作者：
Miller MJ
DOI：
10.1099/00207713-50-1-251
发表时间：
2000-01-01
影响因子：
2.8
作者：
Roos, S;Karner, F;Jonsson, H
通讯作者：
Jonsson, H
DOI：
10.1023/a:1012213728917
发表时间：
2001-10-01
影响因子：
2.6
作者：
Chang, YH;Kim, JK;Park, YH
通讯作者：
Park, YH
影响因子：
4
作者：
Li, X. J.;Yue, L. Y.;Qiao, S. Y.
通讯作者：
Qiao, S. Y.
影响因子：
4.2
作者：
Konstantinov, Sergey R.;Smidt, Hauke;de Vos, Willem M.
通讯作者：
de Vos, Willem M.