Bovine adenovirus serotype 3 utilizes sialic acid as a cellular receptor for virus entry.
Bovine adenovirus serotype 3 utilizes sialic acid as a cellular receptor for virus entry.
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DOI：
10.1016/j.virol.2009.06.029
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发表时间：
2009-09-30
期刊：
影响因子：
3.7
通讯作者：
Mittal, Suresh K.
中科院分区：
文献类型：
--
作者：
Li, Xiaoxin;Bangari, Dinesh S.;Sharma, Anurag;Mittal, Suresh K.
关键词：
牛腺病毒血清3型（BAd3）和猪腺病毒血清3型（PAd3）进入宿主细胞不依赖柯萨奇病毒-腺病毒受体和整合素。研究了唾液酸在BAd3和PAd3进入中的作用。用神经氨酸酶去除唾液酸或用小麦胚芽凝集素阻断唾液酸可显著抑制Madin Darby牛肾细胞BAd3的转导，但不抑制PAd3的转导。Maackia amurensis凝集素或Sambucus nigra （elder）凝集素处理能有效阻断BAd3的转导，表明BAd3利用α(2,3)-连接和α(2,6)-连接的唾液酸作为细胞受体。MDBK细胞的BAd3转导对高碘酸钠、菠萝蛋白酶或胰蛋白酶处理敏感，这表明受体唾液偶联物是一种糖蛋白而不是神经节苷脂。为了确定与BAd3结合的含唾液酸的细胞膜蛋白，进行了病毒覆盖蛋白结合试验（VOPBA），结果显示含有唾液酸的约97和34 kDa细胞膜蛋白与BAd3结合。结果表明唾液酸是BAd3的主要受体。
Bovine adenovirus serotype 3 (BAd3) and porcine adenovirus serotype 3 (PAd3) entry into the host cells is independent of Coxsackievirus -adenovirus receptor and integrins. The role of sialic acid in BAd3 and PAd3 entry was investigated. Removal of sialic acid by neuraminidase, or blocking sialic acid by wheat germ agglutinin lectin significantly inhibited BAd3, but not PAd3, transduction of Madin Darby bovine kidney cells. Maackia amurensis agglutinin or Sambucus nigra (elder) agglutinin treatment efficiently blocked BAd3 transduction suggesting that BAd3 utilized α(2,3)-linked and α(2,6)-linked sialic acid as a cell receptor. BAd3 transduction of MDBK cells was sensitive to sodium periodate, bromelain, or trypsin treatment indicating that the receptor sialoconjugate was a glycoprotein rather than a ganglioside. To determine sialic acid-containing cell membrane proteins that bind to BAd3, virus overlay protein binding assay (VOPBA) was performed and showed that approximately 97 and 34 kDa cell membrane proteins containing sialic acid bind to BAd3. The results suggest sialic acid serves as a primary receptor for BAd3.
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