Role of CgHOG1 in Stress Responses and Glycerol Overproduction of Candida glycerinogenes
Role of CgHOG1 in Stress Responses and Glycerol Overproduction of Candida glycerinogenes
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CgHOG1 在产甘油念珠菌应激反应和甘油过量产生中的作用
DOI：
10.1007/s00284-016-1132-7
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发表时间：
2016-09
影响因子：
2.6
通讯作者：
Zhuge Jian
中科院分区：
文献类型：
--
作者：
Ji Hao;Zhuge Bin;Zong Hong;Lu Xinyao;Fang Huiying;Zhuge Jian
产甘油假丝酵母（Candida glycerinogenes）是一种具有良好的多重胁迫耐受性的甘油生产菌，被认为是一种在极端发酵条件下生产甘油及其衍生物的潜在生物技术宿主。在本研究中，为了评估丝裂原活化蛋白激酶CgHOG 1的多重作用，我们构建了一个二倍体C.以获得CgHOG 1无效突变体。假菌丝生成的CgHOG1突变体在非诱导条件下表示的阻遏作用的形态转变。破坏CgHOG1导致增加的敏感性，渗透压，乙酸和氧化应激，但不参与耐热性。在CgHOG1突变体中，NaCl休克未能刺激细胞内甘油的积累，并且是致命的。此外，CgHOG 1突变体显示出显着延长的生长滞后期在YPD培养基中，没有减少甘油生产，而突变体不能生长在高渗条件下，没有检测到的甘油在肉汤。这些结果表明，CgHOG 1在植物形态建成和多重胁迫耐受中起重要作用。渗透胁迫下的生长和甘油的过量生产严重依赖于CgHOG 1激酶。
Candida glycerinogenes, the glycerol producer with excellent multi-stress tolerances, is considered to be a potential biotechnological host used in the production of glycerol and its derivatives under extreme fermentation conditions. In this study, to evaluate the multiple roles of mitogen-activated protein kinase CgHOG1, we constructed a gene disruption system in the diploid C. glycerinogenes to obtain CgHOG1 null mutant. Pseudohyphae generation of the CgHOG1 mutant under non-inducing condition indicated a repressor role in morphological transitions. Disruption of CgHOG1 resulted in increased sensitivities to osmotic, acetic acid, and oxidative stress but not involved in thermotolerance. In the CgHOG1 mutant, NaCl shock failed to stimulate the accumulation of intracellular glycerol and was fatal. In addition, the CgHOG1 mutant displayed a significant prolonged growth lag phase in YPD medium with no decrease in glycerol production, whereas the mutant cannot grow under hyperosmotic condition with no detectable glycerol in broth. These results suggested that CgHOG1 plays important roles in morphogenesis and multi-stress tolerance. The growth and glycerol overproduction under osmotic stress are heavily dependent on CgHOG1 kinase.
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影响因子：
2.6
作者：
Bouwman, Jildau;Kiewiet, Jose;Bakker, Barbara M.
通讯作者：
Bakker, Barbara M.
影响因子：
5
作者：
J. Zhuge;H. Fang;Zhengliang Wang;D. Chen;Hyuck Jin;H.-L. Gu
通讯作者：
J. Zhuge;H. Fang;Zhengliang Wang;D. Chen;Hyuck Jin;H.-L. Gu
影响因子：
16
作者：
Alepuz, PM;Jovanovic, A;Ammerer, G
通讯作者：
Ammerer, G
DOI：
--
发表时间：
2011-03
期刊：
Wei sheng wu xue bao = Acta microbiologica Sinica
影响因子：
--
作者：
Baoping Song;B. Zhuge;H. Fang;J. Zhuge
通讯作者：
Baoping Song;B. Zhuge;H. Fang;J. Zhuge
影响因子：
3.4
作者：
Cheng Zhang;H. Zong;B. Zhuge;Xinyao Lu;H. Fang;J. Zhuge
通讯作者：
Cheng Zhang;H. Zong;B. Zhuge;Xinyao Lu;H. Fang;J. Zhuge