Transmissible gastroenteritis virus (TGEV)-based vectors with engineered murine tropism express the rotavirus VP7 protein and immunize mice against rotavirus.
Transmissible gastroenteritis virus (TGEV)-based vectors with engineered murine tropism express the rotavirus VP7 protein and immunize mice against rotavirus.
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DOI：
10.1016/j.virol.2010.10.036
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发表时间：
2011-02-05
期刊：
影响因子：
3.7
通讯作者：
Buesa J
中科院分区：
文献类型：
--
作者：
Ribes JM;Ortego J;Ceriani J;Montava R;Enjuanes L;Buesa J
基于表达轮状病毒VP 7蛋白的猪传染性胃肠炎病毒（TGEV）基因组构建冠状病毒载体，以在小鼠模型中免疫和保护针对轮状病毒感染。通过用来自小鼠肝炎病毒（MHV）的同源蛋白替换刺突S蛋白来修改该TGEV衍生载体的向性。将编码轮状病毒VP 7蛋白的基因克隆到冠状病毒cDNA中。将重组病毒载体rTGEVS MHV VP 7接种BALB/c和STAT 1缺陷小鼠，其在肠道中复制并扩散到其他器官，如肝、脾和肺。所有接种的BALB/c小鼠均检测到TGEV特异性抗体，而轮状病毒特异性抗体仅在腹腔免疫后才发现。部分保护轮状病毒诱导的腹泻，实现了哺乳期BALB/c小鼠出生的母鼠免疫表达VP 7的重组病毒，当他们与同型轮状病毒株经口攻击。
A coronavirus vector based on the genome of the porcine transmissible gastroenteritis virus (TGEV) expressing the rotavirus VP7 protein was constructed to immunize and protect against rotavirus infections in a murine model. The tropism of this TGEV-derived vector was modified by replacing the spike S protein with the homologous protein from mouse hepatitis virus (MHV). The rotavirus gene encoding the VP7 protein was cloned into the coronavirus cDNA. BALB/c and STAT1-deficient mice were inoculated with the recombinant viral vector rTGEVS-MHV–VP7, which replicates in the intestine and spreads to other organs such as liver, spleen and lungs. TGEV-specific antibodies were detected in all the inoculated BALB/c mice, while rotavirus-specific antibodies were found only after immunization by the intraperitoneal route. Partial protection against rotavirus-induced diarrhea was achieved in suckling BALB/c mice born to dams immunized with the recombinant virus expressing VP7 when they were orally challenged with the homotypic rotavirus strain.
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