Sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene.
Sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene.
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DOI：
10.1016/0042-6822(86)90102-9
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发表时间：
1986-05
期刊：
影响因子：
3.7
通讯作者：
Brian DA
中科院分区：
文献类型：
--
作者：
Kapke PA;Brian DA
将猪传染性胃肠炎冠状病毒（TGEV）Purdue株20 kb基因组3′端经oligo（dT）引物扩增后，克隆到pUC 9载体的Pst Ⅰ位点上。一个2.0-kb的克隆含有部分poly（A）尾，并使用Maxam和吉尔伯特的化学方法对其进行整体测序。另一个0.7 kb的克隆也含有部分poly（A）尾，并进行了部分测序，以确认基因组最3′端的一级结构。通过对开放阅读框的检查，在3′端1663个碱基序列中鉴定出两个潜在的非重叠基因。第一个基因编码43，426 mol wt的382个氨基酸的蛋白质，其基于大小、化学性质和与其它冠状病毒核衣壳蛋白质的氨基酸序列同源性是表观核衣壳蛋白质。它的5′端侧接至少部分基质蛋白基因。第二个编码一个假设的78个氨基酸的蛋白质的9101摩尔重量是疏水性的两端。还确定了276个碱基的3 '近端非编码序列，并发现poly（A）尾附近的9个核苷酸的保守片段在TGEV、小鼠肝炎冠状病毒和禽传染性支气管炎冠状病毒中很常见。
The 3′ end of the 20-kb genome of the Purdue strain of porcine transmissible gastroenteritis coronavirus (TGEV) was copied into eDNA after priming with oligo(dT) and the double-stranded product was cloned into the PstI site of the pUC9 vector. One clone of 2.0-kb contained part of the poly(A) tail and was sequenced in its entirety using the chemical method of Maxam and Gilbert. Another clone of 0.7 kb also contained part of the poly(A) tail and was sequenced in part to confirm the primary structure of the most 3′ end of the genome. Two potential, nonoverlapping genes were identified within the 3′-terminal 1663-base sequence from an examination of open reading frames. The first gene encodes a 382-amino acid protein of 43,426 mol wt, that is the apparent nucleocapsid protein on the basis of size, chemical properties, and amino acid sequence homology with other coronavirus nucleocapsid proteins. It is flanked on its 5′ side by at least part of the matrix protein gene. The second encodes a hypothetical 78-amino acid protein of 9101 mol wt that is hydrophobic at both ends. A 3′-proximal noncoding sequence of 276 bases was also determined and a conserved stretch of 9 nucleotides near the poly(A) tail was found to be common among TGEV, the mouse hepatitis coronavirus, and the avian infectious bronchitis coronavirus.
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影响因子：
3.8
作者：
GARWES, DJ;POCOCK, DH
通讯作者：
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影响因子：
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