The relationships among spatiotemporal collagen gene expression, histology, and biomechanics following full-length injury in the murine patellar tendon.
The relationships among spatiotemporal collagen gene expression, histology, and biomechanics following full-length injury in the murine patellar tendon.
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DOI：
10.1002/jor.21484
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发表时间：
2012-01
影响因子：
2.8
通讯作者：
Butler, David L.
中科院分区：
文献类型：
--
作者：
Dyment, Nathaniel A.;Kazemi, Namdar;Aschbacher-Smith, Lindsey E.;Barthelery, Nicolas J.;Kenter, Keith;Gooch, Cynthia;Shearn, Jason T.;Wylie, Christopher;Butler, David L.
肌腱损伤是主要的骨科问题，随着人口老龄化而恶化。I型胶原(Col1)和II型胶原(Col2)分别在肌腱中质愈合和肌腱-骨间愈合中发挥重要作用。使用双转基因小鼠，这项研究的目的是时空监测COL1和COL2基因的表达，组织学和生物力学长达8周的全长髌腱损伤。每周分析基因表达和组织学，持续5周，同时在1、2、5和8周测量机械性能。术后1周，愈合区可见疏松的肉芽组织，仅有少量col1表达。COL1表达在2周时达到高峰，但ECM高度无序，细胞增多。3周时，COL1表达减弱，5周时，ECM基本沿腱轴线排列。在愈合中间物或植入处各时间点均未见Col2表达。愈合组织的生物力学在所有时间点都不充分，在8周时达到正常值的48%和63%的极限载荷和硬度。未来的研究将使用张力标记进一步表征愈合中间物质和附着处内的细胞，并将这些结果与正常发育期间的肌腱细胞进行比较。
Tendon injuries are major orthopaedic problems that worsen as the population ages. Type-I (Col1) and type-II (Col2) collagens play important roles in tendon midsubstance and tendon-to-bone insertion healing, respectively. Using double transgenic mice, this study aims to spatiotemporally monitor Col1 and Col2 gene expression, histology and biomechanics up to 8 weeks following a full-length patellar tendon injury. Gene expression and histology were analyzed weekly for up to 5 weeks while mechanical properties were measured at 1, 2, 5, and 8 weeks. At week 1, the healing region displayed loose granulation tissue with little Col1 expression. Col1 expression peaked at 2 weeks, but the ECM was highly disorganized and hypercellular. By 3 weeks, Col1 expression had reduced and by 5 weeks, the ECM was generally aligned along the tendon axis. Col2 expression was not seen in the healing midsubstance or insertion at any time point. The biomechanics of the healing tissue was inadequate at all time points, achieving ultimate loads and stiffnesses of 48% and 63% of normal values by 8 weeks. Future studies will further characterize the cells within the healing midsubstance and insertion using tenogenic markers and compare these results to those of tendon cells during normal development.
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影响因子：
14
作者：
Carpenter, JE;Hankenson, KD
通讯作者：
Hankenson, KD
影响因子：
2.4
作者：
Benjamin, M;Toumi, H;Milz, S
通讯作者：
Milz, S
影响因子：
2.4
作者：
Korvick, DL;Cummings, JF;Butler, DL
通讯作者：
Butler, DL
影响因子：
2.4
作者：
Fung, David T.;Wang, Vincent M.;Andarawis-Puri, Nelly;Basta-Pljakic, Jelena;Li, Yonghui;Laudier, Damien M.;Sun, Hui B.;Jepsen, Karl J.;Schaffler, Mitchell B.;Flatow, Evan L.
通讯作者：
Flatow, Evan L.
影响因子：
4.2
作者：
Jelinsky, Scott A.;Lake, Spencer P.;Archambault, Joanne M.;Soslowsky, Louis J.
通讯作者：
Soslowsky, Louis J.