The Stool Volatile Metabolome of Pre-Term Babies.
The Stool Volatile Metabolome of Pre-Term Babies.
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DOI：
10.3390/molecules26113341
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发表时间：
2021-06-02
期刊：
影响因子：
--
通讯作者：
Probert C
中科院分区：
文献类型：
--
作者：
Frau A;Lett L;Slater R;Young GR;Stewart CJ;Berrington J;Hughes DM;Embleton N;Probert C
生命早期的粪便代谢组很少被研究。我们研究了早产儿出生后第一周内它的演变。研究人员对 51 名妊娠 <32 周的婴儿的多个（n = 152）粪便样本进行了研究。采用顶空固相微萃取气相色谱质谱法对挥发性有机化合物（VOC）进行分析。使用自动质谱解卷积系统 (AMDIS) 和美国国家标准与技术研究院 (NIST) 参考库对数据进行解释。统计分析基于线性混合模型，VOCs数量随时间增加；上升主要发生在第 5 天和第 10 天之间。第 5 天的变化与支链脂肪酸的产物有关。在此之前，代谢组以醛和乙酸为主。剖腹产与真菌来源的分子有一定的关联。这项研究展示了早产儿生命早期代谢组的变化。分娩后 5 天代谢组的变化与肠内喂养的建立和从胎便到粪便的转变同时发生。代谢物的多样性与摄入更多的牛奶有关。
The fecal metabolome in early life has seldom been studied. We investigated its evolution in pre-term babies during their first weeks of life. Multiple (n = 152) stool samples were studied from 51 babies, all <32 weeks gestation. Volatile organic compounds (VOCs) were analyzed by headspace solid phase microextraction gas chromatography mass spectrometry. Data were interpreted using Automated Mass Spectral Deconvolution System (AMDIS) with the National Institute of Standards and Technology (NIST) reference library. Statistical analysis was based on linear mixed modelling, the number of VOCs increased over time; a rise was mainly observed between day 5 and day 10. The shift at day 5 was associated with products of branched-chain fatty acids. Prior to this, the metabolome was dominated by aldehydes and acetic acid. Caesarean delivery showed a modest association with molecules of fungal origin. This study shows how the metabolome changes in early life in pre-term babies. The shift in the metabolome 5 days after delivery coincides with the establishment of enteral feeding and the transition from meconium to feces. Great diversity of metabolites was associated with being fed greater volumes of milk.
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