Acetylome analysis of the feline small intestine following Toxoplasma gondii infection.
Acetylome analysis of the feline small intestine following Toxoplasma gondii infection.
复制标题
弓形虫感染后猫小肠的乙酰组分析
DOI：
10.1007/s00436-020-06880-4
复制
发表时间：
2020-11
影响因子：
2
通讯作者：
He JJ
中科院分区：
文献类型：
--
作者：
Meng YM;Zhai BT;Elsheikha HM;Xie SC;Wang ZX;Zhao Q;Zhu XQ;He JJ
弓形虫是一种能够感染大量温血动物的原生动物寄生虫，并在免疫功能低下的患者中引起严重的健康并发症。T.猫小肠的弓形虫感染对于弓形虫生命周期的完成和传播是至关重要的。刚地。蛋白质乙酰化修饰是一种重要的翻译后修饰，在多种细胞过程的调控中发挥重要作用。因此，了解T.弓形虫重组猫终末宿主的蛋白质乙酰化状态有助于阻止弓形虫的产生和传播。刚地。在此，我们使用亲和富集和高分辨率液相色谱-串联质谱法来分析猫小肠中乙酰组在感染T.弓形虫Prugniuad（Pru）株。分析表明，T.弓形虫在猫肠中诱导蛋白质乙酰化的显著变化。我们确定了2606个独特的赖氨酸乙酰化位点在1357乙酰化蛋白质。在感染的小肠中，334种乙酰化肽的水平下调，而82种乙酰化肽的水平升高。具有差异乙酰化肽的蛋白质在生物能量相关过程中特别富集，如三羧酸循环、氧化磷酸化和氧化还原。这些结果提供了猫小肠在T.弓形虫感染，并应有助于进一步分析乙酰化蛋白在弓形虫发病机制中的作用。最终宿主的弓形虫感染。本文的在线版本（10.1007/s 00436 -020-06880-4）包含补充材料，可供授权用户使用。
Toxoplasma gondii is a protozoan parasite capable of infecting a large number of warm-blooded animals and causes serious health complications in immunocompromised patients. T. gondii infection of the feline small intestine is critical for the completion of the life cycle and transmission of T. gondii. Protein acetylation is an important posttranslational modification, which plays roles in the regulation of various cellular processes. Therefore, understanding of how T. gondii reprograms the protein acetylation status of feline definitive host can help to thwart the production and spread of T. gondii. Here, we used affinity enrichment and high-resolution liquid chromatography with tandem mass spectrometry to profile the alterations of the acetylome in cat small intestine 10 days after infection by T. gondii Prugniuad (Pru) strain. Our analysis showed that T. gondii induced significant changes in the acetylation of proteins in the cat intestine. We identified 2606 unique lysine acetylation sites in 1357 acetylated proteins. The levels of 334 acetylated peptides were downregulated, while the levels of 82 acetylated peptides were increased in the infected small intestine. The proteins with differentially acetylated peptides were particularly enriched in the bioenergetics-related processes, such as tricarboxylic acid cycle, oxidative phosphorylation, and oxidation-reduction. These results provide the first baseline of the global acetylome of feline small intestine following T. gondii infection and should facilitate further analysis of the role of acetylated protein in the pathogenesis of T. gondii infection in its definitive host. The online version of this article (10.1007/s00436-020-06880-4) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
56.9
作者：
Choudhary, Chunaram;Kumar, Chanchal;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
5.4
作者：
Husain, Matloob;Cheung, Chen-Yi
通讯作者：
Cheung, Chen-Yi
Wolf Psort：蛋白质定位预测因子。
影响因子：
14.9
作者：
Horton, Paul;Park, Keun-Joon;Obayashi, Takeshi;Fujita, Naoya;Harada, Hajime;Adams-Collier, C J;Nakai, Kenta
通讯作者：
Nakai, Kenta
猫的急性弓形虫感染诱导由免疫特征主导的组织特异性转录反应
影响因子：
7.3
作者：
Cong W;Dottorini T;Khan F;Emes RD;Zhang FK;Zhou CX;He JJ;Zhang XX;Elsheikha HM;Zhu XQ
通讯作者：
Zhu XQ