Acute Toxoplasma Gondii Infection in Cats Induced Tissue-Specific Transcriptional Response Dominated by Immune Signatures.
Acute Toxoplasma Gondii Infection in Cats Induced Tissue-Specific Transcriptional Response Dominated by Immune Signatures.
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猫的急性弓形虫感染诱导由免疫特征主导的组织特异性转录反应
DOI：
10.3389/fimmu.2018.02403
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发表时间：
2018
影响因子：
7.3
通讯作者：
Zhu XQ
中科院分区：
文献类型：
--
作者：
Cong W;Dottorini T;Khan F;Emes RD;Zhang FK;Zhou CX;He JJ;Zhang XX;Elsheikha HM;Zhu XQ
应用RNA测序技术检测T.弓形虫感染共检测到737个差异表达基因，其中410个表达上调，327个表达下调。肝脏表现出151 DEG，肺（149 DEG），小肠（130 DEG），心脏（123 DEG），脑（104 DEG）和脾脏（80 DEG）-表明组织特异性转录模式。基因本体论和KEGG分析鉴定了在免疫途径中富集的DEG，例如丝氨酸-细胞因子受体相互作用、Jak-STAT信号传导途径、NOD样受体信号传导途径、NF-κ B信号传导途径、MAPK信号传导途径、T细胞受体信号传导途径和胞质DNA传感途径。C-X-C基序趋化因子10（CXCL 10）参与了大多数免疫相关通路。PI 3 K/Akt表达在除脾脏外的所有组织中均下调。磷酸酶，吲哚胺2，3-双加氧酶，Hes家族BHLH转录因子1和鸟苷酸结合蛋白5的基因，在免疫防御中发挥各种作用，在各种猫科动物组织中共表达。多变量K均值聚类分析产生了7个基因簇，其特征在于个体组织特异性的相似基因表达模式，其中肺组织簇具有最大数量的DEG。这些发现表明，在猫的各种组织中存在广泛的免疫防御机制，以对抗急性T。弓形虫感染
RNA-sequencing was used to detect transcriptional changes in six tissues of cats, seven days after T. gondii infection. A total of 737 genes were differentially expressed (DEGs), of which 410 were up-regulated and 327 were down-regulated. The liver exhibited 151 DEGs, lung (149 DEGs), small intestine (130 DEGs), heart (123 DEGs), brain (104 DEGs), and spleen (80 DEGs)-suggesting tissue-specific transcriptional patterns. Gene ontology and KEGG analyses identified DEGs enriched in immune pathways, such as cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, NOD-like receptor signaling pathway, NF-kappa B signaling pathway, MAPK signaling pathway, T cell receptor signaling pathway, and the cytosolic DNA sensing pathway. C-X-C motif chemokine 10 (CXCL10) was involved in most of the immune-related pathways. PI3K/Akt expression was down-regulated in all tissues, except the spleen. The genes for phosphatase, indoleamine 2,3-dioxygenase, Hes Family BHLH Transcription Factor 1, and guanylate-binding protein 5, playing various roles in immune defense, were co-expressed across various feline tissues. Multivariate K-means clustering analysis produced seven gene clusters featuring similar gene expression patterns specific to individual tissues, with lung tissue cluster having the largest number of DEGs. These findings suggest the presence of a broad immune defense mechanism across various tissues in cats against acute T. gondii infection.
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影响因子：
14.2
作者：
Hill, D;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
4.4
作者：
Hehl AB;Basso WU;Lippuner C;Ramakrishnan C;Okoniewski M;Walker RA;Grigg ME;Smith NC;Deplazes P
通讯作者：
Deplazes P
影响因子：
3.2
作者：
Cong W;Meng QF;Song HQ;Zhou DH;Huang SY;Qian AD;Su C;Zhu XQ
通讯作者：
Zhu XQ
DOI：
10.2376/0005-9366-126-216
发表时间：
2013-05-01
影响因子：
0.4
作者：
Atmaca, Hasan Tarik;Dincel, Gungor Cagdas;Kul, Oguz
通讯作者：
Kul, Oguz
弓形虫急性感染后小鼠脾细胞细胞器成分的转录变化
影响因子：
2.1
作者：
He, Jun-Jun;Ma, Jun;Zhu, Xing-Quan
通讯作者：
Zhu, Xing-Quan