Non-natural nucleic acids for synthetic biology.

Non-natural nucleic acids for synthetic biology.
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DOI:
10.1016/j.cbpa.2009.09.030
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发表时间:
2009-12
影响因子:
7.8
通讯作者:
Appella, Daniel H.
Appella, Daniel H.
中科院分区:
生物学2区
文献类型:
--
作者:
Appella, Daniel H.

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基因操作是合成生物学的一个重要方面,但可能会因不希望的核酸酶降解而变得复杂。将非天然核酸转化为基因可以传递对核酸酶的抗性并促进表达。非天然核苷与聚合酶的相容性进行了审查,重点是从过去两年的结果。详细介绍了不同的系统如何在合成生物学中发挥作用。
Genetic manipulation is an important facet of synthetic biology but can be complicated by undesired nuclease degradation. Incorporating non-natural nucleic acids into a gene could convey resistance to nucleases and promote expression. The compatibility of non-natural nucleosides with polymerases is reviewed with a focus on results from the past two years. Details are provided about how the different systems could be useful in synthetic biology.
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