High-Resolution Light Microscopic Characterization of Mouse Spermatogonia1
High-Resolution Light Microscopic Characterization of Mouse Spermatogonia1
复制标题
小鼠精原细胞的高分辨率光学显微镜表征1
作者：
H. Chiarini;L. Russell
摘要采用高分辨率光学显微镜观察了C57 BL/6 J小鼠精原细胞的特征，并对精原细胞的生精周期进行了鉴定。期待每一种精原细胞类型的频率是识别和分类各种细胞类型的主要因素。虽然有许多特征被描述，但精原细胞类型有几个主要的差异。包括As，Apr和Aal精原细胞的组可以主要基于在没有异染色质内衬核膜的情况下异染色质在整个细胞核中的斑点来区分。A1细胞显示细颗粒状染色质遍布细胞核，沿核膜沿着几乎没有异染色质薄片。从A2到A4精原细胞的细胞核边缘含有越来越多的异染色质。中间型精原细胞的异染色质较浅或浅，完全包围细胞核。B型精原细胞沿核膜呈周期性沿着圆形异染色质。灰度直方图的使用允许客观量化的核特性，并显示了逻辑的灰度转移到一个更窄和更暗的配置文件，从四种细胞类型导致A1细胞。区分精原细胞类型的能力是研究正常和异常精子发生中最早的生殖细胞类型的行为和动力学的先决条件。
Abstract Characteristics of spermatogonia were determined in the C57BL/6J strain mouse using high-resolution light microscopy of plastic-embedded tissues and identifying cells during stages of the spermatogenic cycle. The frequency of expecting each spermatogonial cell type was a major factor in identifying and categorizing various cell types. Although numerous characteristics were described, several major differences were noted in spermatogonial cell types. The group comprising As, Apr, and Aal spermatogonia could be differentiated based primarily on mottling of heterochromatin throughout the nucleus in the absence of heterochromatin lining the nuclear envelope. The A1 cells displayed finely granular chromatin throughout the nucleus and virtually no flakes of heterochromatin along the nuclear membrane. The A2 through A4 spermatogonia contained progressively more heterochromatin rimming the nucleus. Intermediate-type spermatogonia displayed flaky or shallow heterochromatin that completely rimmed the nucleus. Type B spermatogonia showed rounded heterochromatin periodically along the nuclear envelope. Use of gray-scale histograms allowed objective quantification of nuclear characteristics and showed a logical shift in the gray scale to a narrower and darker profile, from four cell types leading to A1 cells. The ability to differentiate spermatogonial types is a prerequisite to studying the behavior and kinetics of the earliest of the germ cell types in both normal and abnormal spermatogenesis.
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影响因子：
--
作者：
D. G. Rooij;L. Russell
通讯作者：
D. G. Rooij;L. Russell
影响因子：
--
作者：
WEBER, JE;RUSSELL, LD
通讯作者：
RUSSELL, LD
通过透射电子显微镜表征小鼠精原细胞。
DOI：
10.1530/rep.0.1230567
发表时间：
2002
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Chiarini-Garcia,H;Russell,LD
通讯作者：
Russell,LD
影响因子：
3.2
作者：
LAMMIE, A;DROBNJAK, M;CORDONCARDO, C
通讯作者：
CORDONCARDO, C
影响因子：
3.6
作者：
HIKIM, APS;BARTKE, A;RUSSELL, LD
通讯作者：
RUSSELL, LD