Protein-Inorganic Hybrid Nanoflowers as Efficient Biomimetic Antibiotics in the Treatment of Bacterial Infection.
Protein-Inorganic Hybrid Nanoflowers as Efficient Biomimetic Antibiotics in the Treatment of Bacterial Infection.
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蛋白质-无机杂化纳米花作为治疗细菌感染的有效仿生抗生素
DOI：
10.3389/fchem.2021.681566
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发表时间：
2021
影响因子：
5.5
通讯作者：
Bao X
中科院分区：
文献类型：
--
作者：
Zhou Y;Li Y;Fei Y;Zhang M;Wang S;Li F;Bao X
纳米酶作为新一代抗伤口感染的仿生抗生素已被开发出来。然而，大多数新开发的基于无机粒子或杂化粒子的纳米酶通常来自不相容的原料或不需要的金属盐，这极大地限制了其进一步的生物医学用途。为了克服上述缺点，迫切需要以生物相容的试剂和内源金属物种为原料，开发出具有较强抗菌活性的新型纳米酶。在这里，我们证明了包裹了铜磷蛋白-无机杂化纳米花的牛血清白蛋白具有内在的过氧化物酶样活性，通过纳米酶介导的高毒活性氧(ROS)的产生，可以作为有效的仿生抗生素来对抗细菌感染。我们的纳米花具有极好的过氧化物酶活性，可以在生理条件下有效地杀灭耐药细菌，促进病原体诱导感染后的伤口愈合，并及时避免潜在的组织损伤。对这些纳米花进行的综合毒性检测表明，它们具有较高的生物相容性和良好的生物安全性。我们目前的策略是设计生物安全性高、副作用少的蛋白质-无机杂化纳米酶，为未来基于纳米酶的抗菌平台的开发提供了一种新的范式。
Nanozymes have been developed as new generation of biomimetic antibiotics against wound infection. However, most of new-developed nanozymes based on inorganic particles or hybrid ones usually originate from incompatible raw materials or unwanted metal salts, highly limiting their further biomedical usages. To overcome above drawbacks, it is highly required to develop novel nanozymes with great antibacterial activity by using biocompatible reagents and endogenous metal species as raw materials. Here, we demonstrated that bovine serum albumin enwrapped copper phosphate-based protein-inorganic hybrid nanoflowers possessed intrinsic peroxidase-like activity, which could be used as efficient biomimetic antibiotics against bacterial infection via the nanozyme-mediated generation of high toxic reactive oxygen species (ROS). With the admirable peroxidase-like activity, our nanoflowers could efficiently kill drug-resistance bacteria under physiological conditions, improve the wound healing after pathogen-induced infection, as well as avoid the potential tissue injury in time. Comprehensive toxicity exploration of these nanoflowers indicated their high biocompatibility and excellent biosafety. Our current strategy toward the design of protein-inorganic hybrid nanozymes with high biosafety and few side effects could provide a new paradigm for the development of nanozyme-based antibacterial platform in future.
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影响因子：
29.4
作者：
Wang, Yue;Yang, Yannan;Yu, Chengzhong
通讯作者：
Yu, Chengzhong
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影响因子：
16.6
作者：
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影响因子：
16.6
作者：
Cao, Fangfang;Zhang, Lu;Qu, Xiaogang
通讯作者：
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影响因子：
9.5
作者：
Liu, Yingnan;Guo, Zhirong;Wang, Li
通讯作者：
Wang, Li
有机-无机杂化纳米花的自组装作为自激活串联反应的高效仿生催化剂
影响因子：
4.9
作者：
Huang, Yanyan;Ran, Xiang;Qu, Xiaogang
通讯作者：
Qu, Xiaogang