An almost nontoxic tetrodotoxin analog, 5,6,11-trideoxytetrodotoxin, as an odorant for the grass puffer.
An almost nontoxic tetrodotoxin analog, 5,6,11-trideoxytetrodotoxin, as an odorant for the grass puffer.
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DOI：
10.1038/s41598-022-19355-6
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发表时间：
2022-09-05
影响因子：
4.6
通讯作者：
Abe, Hideki
中科院分区：
文献类型：
--
作者：
Noguchi, Yoshihisa;Suzuki, Takehisa;Matsutani, Keigo;Sakakibara, Ryo;Nakahigashi, Ryota;Adachi, Masaatsu;Nishikawa, Toshio;Abe, Hideki
有毒的河豚含有强烈的神经毒素，河豚毒素（TTX）。虽然TTX被认为是作为一种防御物质，以前的行为研究表明，TTX作为一个有吸引力的信息素，一些有毒的河豚。为了阐明TTX的信息素作用的生理机制，我们研究了草河豚是否可以检测TTX。嗅电图（EOG）结果表明，草河豚的嗅上皮（OE）响应于一种类型的TTX类似物（5，6，11-三脱氧TTX），虽然它不响应TTX。我们还研究了5，6，11-三脱氧TTX对草河豚的吸引作用，通过记录它们在黑暗条件下的游泳行为。草河豚更喜欢呆在水族馆的一侧，其中5，6，11-三脱氧TTX管理，他们的游泳速度下降。此外，气味诱导的标记嗅觉感觉神经元的神经活性标记物（磷酸化细胞外信号调节激酶; pERK）的免疫组化显示，标记的嗅觉感觉神经元定位在周围的区域“胰岛”，其中有被认为是非感觉上皮。已知5，6，11-三脱氧TTX在草河豚中积累，但其毒性比TTX低得多（几乎无毒）。我们的研究结果表明，有毒的河豚可能会积极使用这种TTX类似物，这已经存在于他们的身体与TTX，但其功能是未知的，作为一种气味的化学通讯或有效的TTX积累。
Toxic puffers contain the potent neurotoxin, tetrodotoxin (TTX). Although TTX is considered to serve as a defense substance, previous behavioral studies have demonstrated that TTX acts as an attractive pheromone for some toxic puffers. To elucidate the physiological mechanism of putative pheromonal action of TTX, we examined whether grass puffers Takifugu alboplumbeus can detect TTX. Electroolfactogram (EOG) results suggest that the olfactory epithelium (OE) of grass puffers responded to a type of TTX analog (5,6,11-trideoxyTTX), although it did not respond to TTX. We also examined the attractive action of 5,6,11-trideoxyTTX on grass puffers by recording their swimming behavior under dark conditions. Grass puffers preferred to stay on the side of the aquarium where 5,6,11-trideoxyTTX was administered, and their swimming speed decreased. Additionally, odorant-induced labeling of olfactory sensory neurons by immunohistochemistry against neural activity marker (phosphorylated extracellular signal regulated kinase; pERK) revealed that labeled olfactory sensory neurons were localized in the region surrounding “islets” where there was considered as nonsensory epithelium. 5,6,11-trideoxyTTX has been known to accumulate in grass puffers, but its toxicity is much lower (almost nontoxic) than TTX. Our results suggest that toxic puffers may positively use this TTX analog, which has been present in their body with TTX but whose function was unknown, as an odorant for chemical communication or effective TTX accumulation.
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在饮食中包括planocerid flat虫可以有效地诱捕pufferfish，takifugu niphobles。
影响因子：
4.6
作者：
Itoi S;Ueda H;Yamada R;Takei M;Sato T;Oshikiri S;Wajima Y;Ogata R;Oyama H;Shitto T;Okuhara K;Tsunashima T;Sawayama E;Sugita H
通讯作者：
Sugita H
影响因子：
4.6
作者：
Biechl, Daniela;Tietje, Kristin;Wullimann, Mario F.
通讯作者：
Wullimann, Mario F.
影响因子：
2.8
作者：
KODAMA, M;SATO, S;AIDA, K
通讯作者：
AIDA, K
影响因子：
5.4
作者：
Jang, Jun-Ho;Lee, Jong-Soo;Yotsu-Yamashita, Mari
通讯作者：
Yotsu-Yamashita, Mari
影响因子：
2.8
作者：
Doving, Kjell B.;Hansson, Kenth-Arne;Hamdani, El Hassan
通讯作者：
Hamdani, El Hassan