Genome-Wide Identification and Transcriptome-Based Expression Profile of Cuticular Protein Genes in Antheraea pernyi.
Genome-Wide Identification and Transcriptome-Based Expression Profile of Cuticular Protein Genes in Antheraea pernyi.
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DOI：
10.3390/ijms24086991
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发表时间：
2023-04-10
影响因子：
5.6
通讯作者：
Liu, Yanqun
中科院分区：
文献类型：
--
作者：
Fu, Xin;Chen, Miaomiao;Xia, Runxi;Li, Xinyu;Li, Qun;Li, Yuping;Cao, Huiying;Liu, Yanqun
柞蚕（Antheraea pernyi）是柞蚕科最著名的食用和产丝野生蚕之一。结构角质层蛋白（CPs）是昆虫角质层的主要成分。本文通过对两种家蚕幼虫表皮/被皮（以下为表皮）和部分非表皮组织/器官的转录组学数据，分析了两种家蚕基因组中CPs的表达模式，并将其与鳞翅目模式种家蚕进行了比较。共鉴定出217个CPs，与家蚕（236个）相当，其中CPLCP和CPG家族是造成两种家蚕数量差异的主要原因。结果表明，柞蚕5龄幼虫表皮中r -2基因的表达量高于家蚕，而前胸腺中r -2基因的表达量低于家蚕，说明两种幼虫表皮和前胸腺硬度的差异可能是由r -2基因的表达量引起的。我们还发现，家蚕5龄体中和体和前胸腺中CP基因的表达量高于幼虫表皮。我们的工作为土星科CP基因的功能研究提供了一个总体框架。
Antheraea pernyi is one of the most famous edible and silk-producing wild silkworms of Saturniidae. Structural cuticular proteins (CPs) are the primary component of insect cuticle. In this paper, the CPs in the genome of A. pernyi were identified and compared with those of the lepidopteran model species Bombyx mori, and expression patterns were analyzed based on the transcriptomic data from the larval epidermis/integument (epidermis in the following) and some non-epidermis tissues/organs of two silkworm species. A total of 217 CPs was identified in the A. pernyi genome, a comparable number to B. mori (236 CPs), with CPLCP and CPG families being the main contribution to the number difference between two silkworm species. We found more RR-2 genes expressed in the larval epidermis of fifth instar of A. pernyi than B. mori, but less RR-2 genes expressed in the prothoracic gland of A. pernyi than B. mori, which suggests that the hardness difference in the larval epidermis and prothoracic gland between the two species may be caused by the number of RR-2 genes expressed. We also revealed that, in B. mori, the number of CP genes expressed in the corpus allatum and prothoracic gland of fifth instar was higher than that in the larval epidermis. Our work provided an overall framework for functional research into the CP genes of Saturniidae.
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影响因子：
3.8
作者：
Andersen, SO
通讯作者：
Andersen, SO
影响因子：
2
作者：
Fang, Fujin;Wang, Weijie;Zhu, Changliang
通讯作者：
Zhu, Changliang
影响因子：
3.8
作者：
Ioannidou, Zoi S.;Theodoropoulou, Margarita C.;Papandreou, Nikos C.;Willis, Judith H.;Hamodrakas, Stavros J.
通讯作者：
Hamodrakas, Stavros J.
影响因子：
3.8
作者：
Andersen, SO
通讯作者：
Andersen, SO
柞蚕（Saturniidae，鳞翅目）染色体规模基因组组装
影响因子：
7.7
作者：
Duan, Jianping;Li, Ying;Wu, Xiangwei
通讯作者：
Wu, Xiangwei