Deletion of the oligopeptide transporter Lmo2193 decreases the virulence of Listeria monocytogenes.
Deletion of the oligopeptide transporter Lmo2193 decreases the virulence of Listeria monocytogenes.
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寡肽转运蛋白 Lmo2193 的缺失降低了单核细胞增生李斯特氏菌的毒力
DOI：
10.4142/jvs.2020.21.e88
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发表时间：
2020-11
影响因子：
1.8
通讯作者：
Ma X
中科院分区：
文献类型：
--
作者：
Li H;Qiao Y;Du D;Wang J;Ma X
单核细胞增生李斯特菌是一种革兰氏阳性菌，主要在免疫功能低下的宿主中引起李斯特菌病。它还可导致食源性疾病暴发，并具有适应各种环境的能力。革兰氏阳性菌中的肽摄取是由寡肽通透酶（Opp）实现的，这一过程依赖于OppD和F对ATP的水解。此前，一种假定的蛋白质Lmo2193被预测为OppD，但对于OppD在单核细胞增生李斯特菌的主要过程（如生长、毒力和生物膜形成）中的作用知之甚少。
为了确定单核细胞增生李斯特菌的毒力特性是否与OppD有关。
在本研究中，构建了单核细胞增生李斯特菌的lmo2193基因缺失株和互补株，并与野生型菌株在以下方面进行了比较：黏附性、侵袭能力、细胞内存活、增殖、对小鼠的半数致死剂量（LD50）以及小鼠肝脏、脾脏和大脑中的细菌数量。
结果表明，缺失株的毒力分别比野生型菌株和互补株高1.34个和0.5个数量级。Lmo2193的功能被预测并验证为来自ATP酶超家族的OppD。lmo2193的缺失影响了单核细胞增生李斯特菌的正常生长，降低了其在细胞和小鼠中的毒力，并影响了其形成生物膜的能力。
寡肽转运蛋白Lmo2193的缺失降低了单核细胞增生李斯特菌的毒力。这些影响可能与OppD的功能有关，这为单核细胞增生李斯特菌中寡肽转运蛋白的调控提供了新的视角。
Listeria monocytogenes is a gram-positive bacterium that causes listeriosis mainly in immunocompromised hosts. It can also cause foodborne outbreaks and has the ability to adapt to various environments. Peptide uptake in gram-positive bacteria is enabled by oligopeptide permeases (Opp) in a process that depends on ATP hydrolysis by OppD and F. Previously a putative protein Lmo2193 was predicted to be OppD, but little is known about the role of OppD in major processes of L. monocytogenes, such as growth, virulence, and biofilm formation. To determine whether the virulence traits of L. monocytogenes are related to OppD. In this study, lmo2193 gene deletion and complementation strains of L. monocytogenes were generated and compared with a wild-type strain for the following: adhesiveness, invasion ability, intracellular survival, proliferation, 50% lethal dose (LD50) to mice, and the amount bacteria in the mouse liver, spleen, and brain. The results showed that virulence of the deletion strain was 1.34 and 0.5 orders of magnitude higher than that of the wild-type and complementation strains, respectively. The function of Lmo2193 was predicted and verified as OppD from the ATPase superfamily. Deletion of lmo2193 affected the normal growth of L. monocytogenes, reduced its virulence in cells and mice, and affected its ability to form biofilms. Deletion of the oligopeptide transporter Lmo2193 decreases the virulence of L. monocytogenes. These effects may be related to OppD's function, which provides a new perspective on the regulation of oligopeptide transporters in L. monocytogenes.
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影响因子：
4.2
作者：
Hopfe M;Dahlmanns T;Henrich B
通讯作者：
Henrich B
影响因子：
5.8
作者：
Lecuit, Marc
通讯作者：
Lecuit, Marc
影响因子：
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作者：
Orchard, SS;Goodrich-Blair, H
通讯作者：
Goodrich-Blair, H
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作者：
Sievers, Susanne;Lund, Anja;Kallipolitis, Birgitte Haahr
通讯作者：
Kallipolitis, Birgitte Haahr
DOI：
10.1099/ijs.0.052720-0
发表时间：
2014-06-01
影响因子：
2.8
作者：
den Bakker, Henk C.;Warchocki, Steven;Wiedmann, Martin
通讯作者：
Wiedmann, Martin