Colonization on root surface by a phenanthrene-degrading endophytic bacterium and its application for reducing plant phenanthrene contamination.
Colonization on root surface by a phenanthrene-degrading endophytic bacterium and its application for reducing plant phenanthrene contamination.
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菲降解内生细菌在根表面的定殖及其在减少植物菲污染中的应用
DOI：
10.1371/journal.pone.0108249
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gao Y
中科院分区：
文献类型：
--
作者：
Liu J;Liu S;Sun K;Sheng Y;Gu Y;Gao Y
从生长在多环芳烃（PAHs）污染土壤中的看麦娘（Alopecurus aequalis Sobol）中分离出一株菲降解内生细菌Pn2。基于形态学、生理特性以及16S rRNA基因序列，它被鉴定为马赛菌属（Massilia sp.）。菌株Pn2在初始pH为7.0、温度为30°C的条件下，能在48小时内降解无机盐培养基（MSM）中超过95%的菲（150 mg·L⁻¹）。Pn2能在含有一系列其他多环芳烃（包括萘、苊、蒽和芘）的MSM平板上良好生长，并对它们有不同程度的降解。Pn2还能在黑麦草（Lolium multiflorum Lam）根表面定殖，侵入其内部根组织并转移到植物地上部分。在水培生长条件下，用含2 mg·L⁻¹菲的霍格兰溶液处理，与未接种内生菌的处理相比，接种内生菌Pn2的黑麦草根部和地上部分的菲浓度分别降低了54%和57%。菌株Pn2可能是一种新型且有用的细菌资源，可通过降解植物体内的多环芳烃来消除污染环境中植物的多环芳烃污染。此外，我们为通过内生菌控制植物对多环芳烃的吸收提供了新的视角。
A phenanthrene-degrading endophytic bacterium, Pn2, was isolated from Alopecurus aequalis Sobol grown in soils contaminated with polycyclic aromatic hydrocarbons (PAHs). Based on morphology, physiological characteristics and the 16S rRNA gene sequence, it was identified as Massilia sp. Strain Pn2 could degrade more than 95% of the phenanthrene (150 mg·L−1) in a minimal salts medium (MSM) within 48 hours at an initial pH of 7.0 and a temperature of 30°C. Pn2 could grow well on the MSM plates with a series of other PAHs, including naphthalene, acenaphthene, anthracene and pyrene, and degrade them to different degrees. Pn2 could also colonize the root surface of ryegrass (Lolium multiflorum Lam), invade its internal root tissues and translocate into the plant shoot. When treated with the endophyte Pn2 under hydroponic growth conditions with 2 mg·L−1 of phenanthrene in the Hoagland solution, the phenanthrene concentrations in ryegrass roots and shoots were reduced by 54% and 57%, respectively, compared with the endophyte-free treatment. Strain Pn2 could be a novel and useful bacterial resource for eliminating plant PAH contamination in polluted environments by degrading the PAHs inside plants. Furthermore, we provide new perspectives on the control of the plant uptake of PAHs via endophytic bacteria.
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