In utero exposure to a low concentration of diesel exhaust affects spontaneous locomotor activity and monoaminergic system in male mice.
In utero exposure to a low concentration of diesel exhaust affects spontaneous locomotor activity and monoaminergic system in male mice.
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DOI：
10.1186/1743-8977-7-7
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发表时间：
2010-03-23
影响因子：
10
通讯作者：
Takeda K
中科院分区：
文献类型：
--
作者：
Suzuki T;Oshio S;Iwata M;Saburi H;Odagiri T;Udagawa T;Sugawara I;Umezawa M;Takeda K
流行病学研究表明，悬浮颗粒物 (SPM) 会对健康产生不利影响，例如呼吸系统疾病和心血管疾病，而汽车柴油机尾气颗粒是悬浮颗粒物的主要来源。据报道，新生儿和成人接触柴油机尾气会损害中枢神经系统（CNS）并引起行为改变。最近，我们重点关注产前接触柴油废气对中枢神经系统的影响。在这项研究中，我们研究了产前暴露于低浓度柴油机尾气对行为和单胺能神经元系统的影响。评估中枢神经系统的自发运动活动（SLA）和单胺水平。在妊娠第 2-16 天，小鼠在产前暴露于低浓度柴油机尾气 (171 μg DEP/m3) 中，每天 8 小时。通过分析与温度相关的红外线的释放，对 4 周龄小鼠的 SLA 进行了 3 天的评估。 5周龄时，处死小鼠并将大脑用于高效液相色谱（HPLC）分析。暴露于低浓度柴油废气的小鼠在暴露的前 60 分钟内表现出 SLA 下降。在整个测试期间，与对照小鼠相比，产前暴露于柴油废气的小鼠的每日 SLA 有所下降，并且在开灯时每 3 小时内的 SLA 有所下降。与对照组相比，暴露组前额皮质（PFC）的神经递质水平，包括多巴胺和去甲肾上腺素，有所增加。 PFC 中多巴胺和去甲肾上腺素的代谢物也增加。在暴露组中，中枢神经系统各个区域（包括纹状体）的多巴胺和去甲肾上腺素的神经递质周转率（神经元活动指数）均下降。各组之间的血清皮质酮水平没有差异。数据表明，产前暴露于柴油废气的小鼠的 SLA 降低是由于 PFC 中多巴胺的促进释放所致。这些结果表明，子宫内的小鼠暴露于低浓度的柴油尾气会降低 SLA 并改变大脑多个区域的神经化学单胺代谢。
Epidemiological studies have suggested that suspended particulate matter (SPM) causes detrimental health effects such as respiratory and cardiovascular diseases, and that diesel exhaust particles from automobiles is a major contributor to SPM. It has been reported that neonatal and adult exposure to diesel exhaust damages the central nervous system (CNS) and induces behavioral alteration. Recently, we have focused on the effects of prenatal exposure to diesel exhaust on the CNS. In this study, we examined the effects of prenatal exposure to low concentration of diesel exhaust on behaviour and the monoaminergic neuron system. Spontaneous locomotor activity (SLA) and monoamine levels in the CNS were assessed. Mice were exposed prenatally to a low concentration of diesel exhaust (171 μg DEP/m3) for 8 hours/day on gestational days 2-16. SLA was assessed for 3 days in 4-week-old mice by analysis of the release of temperature-associated infrared rays. At 5 weeks of age, the mice were sacrificed and the brains were used for analysis by high-performance liquid chromatography (HPLC). Mice exposed to a low concentration of diesel exhaust showed decreased SLA in the first 60 minutes of exposure. Over the entire test period, the mice exposed prenatally to diesel exhaust showed decreased daily SLA compared to that in control mice, and the SLA in each 3 hour period was decreased when the lights were turned on. Neurotransmitter levels, including dopamine and noradrenaline, were increased in the prefrontal cortex (PFC) in the exposure group compared to the control group. The metabolites of dopamine and noradrenaline also increased in the PFC. Neurotransmitter turnover, an index of neuronal activity, of dopamine and noradrenaline was decreased in various regions of the CNS, including the striatum, in the exposure group. The serum corticosterone level was not different between groups. The data suggest that decreased SLA in mice exposed prenatally to diesel exhaust is due to facilitated release of dopamine in the PFC. These results indicate that exposure of mice in utero to a low concentration of diesel exhaust decreases SLA and alters the neurochemical monoamine metabolism of several regions of the brain.
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影响因子：
37.8
作者：
Pope, CA;Burnett, RT;Godleski, JJ
通讯作者：
Godleski, JJ
影响因子：
2.9
作者：
IMPERATO, A;PUGLISIALLEGRA, S;ANGELUCCI, L
通讯作者：
ANGELUCCI, L
影响因子：
3.7
作者：
Furuta, C;Li, CM;Taya, K
通讯作者：
Taya, K
DOI：
10.1016/0165-3806(83)90105-0
发表时间：
1983-01-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
HEFFNER, TG;HELLER, A;SEIDEN, LS
通讯作者：
SEIDEN, LS
影响因子：
3.2
作者：
OADES, RD;TAGHZOUTI, K;LEMOAL, M
通讯作者：
LEMOAL, M