Proteomic Dissection of Seed Germination and Seedling Establishment in Brassica napus.
Proteomic Dissection of Seed Germination and Seedling Establishment in Brassica napus.
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甘蓝型油菜种子萌发和幼苗建立的蛋白质组学解析
DOI：
10.3389/fpls.2016.01482
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发表时间：
2016
影响因子：
5.6
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Gu J;Chao H;Gan L;Guo L;Zhang K;Li Y;Wang H;Raboanatahiry N;Li M
种子萌发成功和正常幼苗的建立是植物物种繁殖的关键决定因素。目前，利用蛋白质组学方法对甘蓝型油菜种子萌发的遗传调控研究较少。本研究采用差异荧光双向凝胶电泳技术研究了B中种子萌发过程中蛋白质的表达模式。油菜。113个差异表达蛋白（DEPs）主要参与贮藏（23.4%），能量代谢（18.9%），蛋白质代谢（16.2%），防御/疾病（12.6%），种子成熟（11.7%），碳水化合物代谢（4.5%），脂质代谢蛋白质组学分析显示，蛋白质组学分析主要涉及蛋白质组学（4.5%）、氨基酸代谢（3.6%）、细胞生长/分裂（3.6%）和一些功能不明确的蛋白质组学（2.7%）。在B中报道的与种子发芽和活力相关的数量性状的连锁不平衡区域内或附近，鉴定出11个DEPs对应的17个基因。在以往的研究中，蛋白质的表达模式表明，在种子萌发过程中，异养代谢可以被激活，防御机制的启动可能开始于种子萌发。这些发现将有助于更深入地了解种子贮藏储备的动员和萌发过程中的调控机制B。油菜。
The success of seed germination and establishment of a normal seedling are key determinants of plant species propagation. At present, only a few studies have focused on the genetic control of seed germination by using a proteomic approach in Brassica napus. In the present study, the protein expression pattern of seed germination was investigated using differential fluorescence two-dimensional gel electrophoresis in B. napus. One hundred and thirteen differentially expressed proteins (DEPs) that were mainly involved in storage (23.4%), energy metabolism (18.9%), protein metabolism (16.2%), defense/disease (12.6%), seed maturation (11.7%), carbohydrate metabolism (4.5%), lipid metabolism (4.5%), amino acids metabolism (3.6%), cell growth/division (3.6%), and some unclear functions (2.7%) were observed by proteomic analysis. Seventeen genes corresponding to 11 DEPs were identified within or near the associated linkage disequilibrium regions related to seed germination and vigor quantitative traits reported in B. napus in previous studies. The expression pattern of proteins showed that heterotrophic metabolism could be activated in the process of seed germination and that the onset of defense mechanisms might start during seed germination. These findings will help generate a more in-depth understanding of the mobilization of seed storage reserves and regulation mechanisms of the germination process in B. napus.
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影响因子：
3.7
作者：
Han C;Yin X;He D;Yang P
通讯作者：
Yang P
影响因子：
7.4
作者：
Bassel, George W.;Fung, Pauline;Cutler, Sean R.
通讯作者：
Cutler, Sean R.
影响因子：
3.4
作者：
Giavalisco, P;Nordhoff, E;Gobom, J
通讯作者：
Gobom, J
影响因子：
3.4
作者：
He, Dongli;Han, Chao;Yang, Pingfang
通讯作者：
Yang, Pingfang
影响因子：
3.1
作者：
Fulnecek, Jaroslav;Matyasek, Roman;Kovarik, Ales
通讯作者：
Kovarik, Ales