Chemical sintering generates uniform porous hyaluronic acid hydrogels.
Chemical sintering generates uniform porous hyaluronic acid hydrogels.
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DOI：
10.1016/j.actbio.2013.10.002
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发表时间：
2014-01
影响因子：
9.7
通讯作者：
Segura, Tatiana
中科院分区：
文献类型：
--
作者：
Cam, Cynthia;Segura, Tatiana
用于组织修复的支架的植入已经遇到了有限的成功，主要是由于不能在构造内实现血管化。许多策略已经转向将孔并入这些支架中以促进快速细胞浸润和随后的血管向内生长。我们利用一种有效的化学烧结技术，以创建一个均匀的网络的聚甲基丙烯酸甲酯（PMMA）微球多孔透明质酸水凝胶的形成。由化学烧结产生的多孔水凝胶具有与常用的非烧结和热烧结技术相当的孔均匀性和互连性。此外，在细胞活力、扩散、体外增殖以及体内细胞侵袭方面观察到对由每种烧结方法产生的多孔水凝胶的类似细胞响应。我们建议使用稀释的丙酮溶液作为替代方法来产生多孔透明质酸水凝胶的PMMA微球的化学烧结，因为它需要与目前使用的非烧结或热烧结技术相等或少十倍的处理时间。
Implantation of scaffolds for tissue repair has been met with limited success primarily due to the inability to achieve vascularization within the construct. Many strategies have shifted to incorporate pores into these scaffolds to encourage rapid cellular infiltration and subsequent vascular ingrowth. We utilized an efficient chemical sintering technique to create a uniform network of polymethyl methacrylate (PMMA) microspheres for porous hyaluronic acid hydrogel formation. The porous hydrogels generated from chemical sintering possessed comparable pore uniformity and interconnectivity as the commonly used non- and heat sintering techniques. Moreover, similar cell response to the porous hydrogels generated from each sintering approach was observed in cell viability, spreading, proliferation in vitro, as well as, cellular invasion in vivo. We propose chemical sintering of PMMA microspheres using a dilute acetone solution as an alternative method to generating porous hyaluronic acid hydrogels since it requires equal or ten-fold less processing time as the currently used non-sintering or heat sintering technique, respectively.
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