Action of x-rays on mammalian cells.
Action of x-rays on mammalian cells.
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DOI：
10.1084/jem.103.5.653
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发表时间：
1956-05-01
期刊：
影响因子：
--
通讯作者：
MARCUS PI
中科院分区：
文献类型：
--
作者：
PUCK TT;MARCUS PI
X射线照射的影响已被定量研究的条件下，使100%的未照射的细胞在隔离，形成宏观集落的人宫颈癌（HeLa）的单细胞。这种技术消除了由于大细胞群体成员之间的相互作用而产生的复杂性。单细胞的存活率（定义为在15天内形成肉眼可见集落的能力）在相对于X射线剂量作图时产生典型的2次命中曲线。最初的剂量肩延伸到约75 r，之后获得线性对数存活率，其中将存活者减少到37%所需的剂量为96 r。这种辐射敏感性比任何已研究过同等功能的微生物的辐射敏感性大几十到几百倍。证据，虽然不是证据，提出的致命影响是由于辐射引起的遗传缺陷，但是，不能是一个简单的单一基因失活。作用位点可能是染色体。从100 μ l剂量开始，或可能更早，辐射的生长延迟效应是可见的。细胞的繁殖能力已被破坏的剂量低于800 r，仍然可以繁殖几倍。在更高的剂量下，甚至单个细胞分裂也被阻止。在任何剂量的辐射中，大部分被杀死的细胞都会产生一个或多个巨细胞。这些代谢活跃，生长到巨大的比例，但在实验条件下从未繁殖。描述了制备大群巨细胞的方法。这些巨人特别容易受到病毒的影响。一些被照射的细胞从平板上消失，可能是由于解体。辐射的这种作用是迄今为止效率最低的，因为即使在10，000 r之后，原始细胞接种物的5%至10%仍可以恢复为巨人。
The effects of x-irradiation have been quantitatively studied on single cells of a human cervical carcinoma (HeLa) under conditions such that 100 per cent of the unirradiated cells reproduce in isolation to form macroscopic colonies. This technique eliminates complexities due to interactions of members of large cell populations. Survival of single cells (defined as the ability to form a macroscopic colony within 15 days) yields a typical 2 hit curve when plotted against x-ray dose. The initial shoulder extends to about 75 r, after which a linear logarithmic survival rate is obtained, in which the dose needed to reduce survivors to 37 per cent is 96 r. This radiation sensitivity is tens to hundreds of times greater than that of any microorganism for which the equivalent function bas been studied. Evidence, though not proof, is presented that the lethal effect is due to a radiation-induced genetic defect which, however, cannot be a simple single gene inactivation. The locus of the action could be chromosomal. Beginning at doses of 100 r, or possibly earlier, growth-delaying effects of radiation are visible. Cells in which the ability to reproduce has been destroyed by doses below 800 r, can still multiply several times. At higher doses even a single cell division is precluded. A large proportion of the cells killed by radiation at any dose gives rise to one or more giant cells. These metabolize actively, grow to huge proportions but never reproduce under the experimental conditions employed. Methods of preparing large populations of giant cells are described. These giants are particularly susceptible to virus action. Some of the irradiated cells disappear from the plate, presumably by disintegration. This action of radiation is by far the least efficient, since even after 10,000 r, 5 to 10 per cent of the original cell inoculum is recoverable as giants.
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影响因子：
64.8
作者：
LUCKE, WH;SARACHEK, A
通讯作者：
SARACHEK, A
DOI：
10.1073/pnas.35.12.696
发表时间：
1949-01-01
影响因子：
11.1
作者：
ATWOOD, KC;NORMAN, A
通讯作者：
NORMAN, A
DOI：
10.1073/pnas.41.7.432
发表时间：
1955-01-01
影响因子：
11.1
作者：
PUCK, TT;MARCUS, PI
通讯作者：
MARCUS, PI
DOI：
10.1101/sqb.1951.016.01.024
发表时间：
1951-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
RUSSELL, WL
通讯作者：
RUSSELL, WL
DOI：
10.1084/jem.103.2.273
发表时间：
1956-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
PUCK TT;MARCUS PI;CIECIURA SJ
通讯作者：
CIECIURA SJ