DNA Computing Systems Activated by Electrochemically-triggered DNA Release from a Polymer-brush-modified Electrode Array.
DNA Computing Systems Activated by Electrochemically-triggered DNA Release from a Polymer-brush-modified Electrode Array.
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DNA计算系统是由电化学触发的DNA从聚合物刷子修饰的电极阵列释放而激活的。
DOI：
10.1002/elan.201600389
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发表时间：
2017-03
期刊：
影响因子：
3
通讯作者：
Katz E
中科院分区：
文献类型：
--
作者：
Gamella M;Zakharchenko A;Guz N;Masi M;Minko S;Kolpashchikov DM;Iken H;Poghossian A;Schöning MJ;Katz E
四个独立布线的氧化铟锡（ITO）电极的阵列用于电化学刺激的DNA释放和基于DNA的身份、AND和XOR逻辑门的激活。将单链DNA分子负载在聚（N，N-二甲基氨基乙基甲基丙烯酸酯）（PDMAEMA）/聚（甲基丙烯酸）（PMAA）混合刷上，该刷共价连接到ITO电极。当聚合物刷由于PDMAE-MA中叔氨基的质子化而带正电时，在pH 5.0下进行DNA沉积，从而导致带负电的DNA的静电吸引。通过在-1.0 V（相对于Ag/AgCl参比）下进行电解，电化学氧还原导致氢离子的消耗和电极表面附近的局部pH值升高。由于PMAA的羧基的解离，该过程导致聚合物刷再充电至负状态，从而排斥带负电荷的DNA并将其从电极表面释放。DNA释放以各种组合从阵列组件中的不同电极进行。释放的DNA作为布尔逻辑门激活的输入信号。开发的系统代表了DNA计算的一个进步，首次将DNA化学过程与电子输入信号相结合。
An array of four independently wired indium tin oxide (ITO) electrodes was used for electrochemically stimulated DNA release and activation of DNA-based Identity, AND and XOR logic gates. Single-stranded DNA molecules were loaded on the mixed poly(N,N-di-methylaminoethyl methacrylate) (PDMAEMA)/poly-(methacrylic acid) (PMAA) brush covalently attached to the ITO electrodes. The DNA deposition was performed at pH 5.0 when the polymer brush is positively charged due to protonation of tertiary amino groups in PDMAE-MA, thus resulting in electrostatic attraction of the negatively charged DNA. By applying electrolysis at −1.0 V(vs. Ag/AgCl reference) electrochemical oxygen reduction resulted in the consumption of hydrogen ions and local pH increase near the electrode surface. The process resulted in recharging the polymer brush to the negative state due to dissociation of carboxylic groups of PMAA, thus repulsing the negatively charged DNA and releasing it from the electrode surface. The DNA release was performed in various combinations from different electrodes in the array assembly. The released DNA operated as input signals for activation of the Boolean logic gates. The developed system represents a step forward in DNA computing, combining for the first time DNA chemical processes with electronic input signals.
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影响因子：
16.6
作者：
Cornett, Evan M;Campbell, Eleanor A;Gulenay, George;Peterson, Evan;Bhaskar, Neha;Kolpashchikov, Dmitry M
通讯作者：
Kolpashchikov, Dmitry M
基于三螺旋分子开关的DNA比色逻辑门
影响因子：
3.7
作者：
Gao, Wan;Zhang, Li;Qiu, Jian-Ding
通讯作者：
Qiu, Jian-Ding
影响因子：
3.2
作者：
Gerasimova, Yulia V.;Cornett, Evan;Kolpashchikov, Dmitry M.
通讯作者：
Kolpashchikov, Dmitry M.
影响因子：
4.1
作者：
Gerasimova, Yulia V.;Kolpashchikov, Dmitry M.
通讯作者：
Kolpashchikov, Dmitry M.
影响因子：
2.9
作者：
Fratto, Brian E.;Katz, Evgeny
通讯作者：
Katz, Evgeny