Metabolome and Transcriptome Analyses of Cucurbitacin Biosynthesis in Luffa (Luffa acutangula).
Metabolome and Transcriptome Analyses of Cucurbitacin Biosynthesis in Luffa (Luffa acutangula).
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丝瓜 (Luffa acutangula) 葫芦素生物合成的代谢组和转录组分析
DOI：
10.3389/fpls.2022.886870
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发表时间：
2022
影响因子：
5.6
通讯作者：
Wu, Haibin
中科院分区：
文献类型：
--
作者：
Zhao, Gangjun;Wang, Meng;Luo, Caixia;Li, Junxing;Gong, Hao;Zheng, Xiaoming;Liu, Xiaoxi;Luo, Jianning;Wu, Haibin
葫芦素是一种极苦的化合物，主要存在于丝瓜所属的葫芦科植物中。然而，目前还没有对丝瓜果实中葫芦素生物合成的全面分析。因此，本研究分析了丝瓜的苦味（WM709）和非苦味（S1174）基因型，通过整合代谢组和转录组分析来揭示葫芦素生物合成的潜在机制。共检测出422种代谢物，包括维生素、必需氨基酸、抗氧化剂、抗肿瘤物质等。其中，131 种代谢物在苦味 (WM709) 和非苦味 (S1174) 丝瓜果实之间表现出显着差异。苦丝瓜中异葫芦素B、葫芦素D、23,24-二氢葫芦素E、葫芦素F的含量显着高于非苦丝瓜。转录组分析表明，葫芦素生物合成途径的 Bi、细胞色素 P450s (CYP450s) 和酰基转移酶 (ACT) 显着上调。此外，干旱胁迫和脱落酸（ABA）激活了葫芦素生物合成途径的基因。此外，双荧光素酶报告基因和酵母单杂交测定表明，ABA 响应元件结合因子 1 (AREB1) 与 Bi 启动子结合以激活 Bi 表达。丝瓜和黄瓜基因组的比较分析表明，Bi、CYP450和ACT位于保守的同线性位点，并形成葫芦素生物合成簇。该研究为葫芦素生物合成途径的主要基因和代谢物提供了重要的见解，加深了对丝瓜葫芦素生物合成调控机制的理解。
Cucurbitacins are extremely bitter compounds mainly present in Cucurbitaceae, where Luffa belongs. However, there is no comprehensive analysis of cucurbitacin biosynthesis in Luffa fruit. Therefore, this study analyzed bitter (WM709) and non-bitter (S1174) genotypes of Luffa to reveal the underlying mechanism of cucurbitacin biosynthesis by integrating metabolome and transcriptome analyses. A total of 422 metabolites were detected, including vitamins, essential amino acids, antioxidants, and antitumor substances. Of these, 131 metabolites showed significant differences between bitter (WM709) and non-bitter (S1174) Luffa fruits. The levels of isocucurbitacin B, cucurbitacin D, 23,24-dihydro cucurbitacin E, cucurbitacin F were significantly higher in bitter than in non-bitter Luffa. Transcriptome analysis showed that Bi, cytochromes P450s (CYP450s), and acyltransferase (ACT) of the cucurbitacin biosynthesis pathway, were significantly up-regulated. Moreover, drought stress and abscisic acid (ABA) activated genes of the cucurbitacin biosynthesis pathway. Furthermore, dual-luciferase reporter and yeast one-hybrid assays demonstrated that ABA-response element binding factor 1 (AREB1) binds to the Bi promoter to activate Bi expression. Comparative analysis of the Luffa and cucumber genomes showed that Bi, CYP450s, and ACT are located in the conserved syntenic loci, and formed a cucurbitacin biosynthesis cluster. This study provides important insights into major genes and metabolites of the cucurbitacin biosynthetic pathway, deepening the understanding of regulatory mechanisms of cucurbitacin biosynthesis in Luffa.
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影响因子：
14.9
作者：
The Gene Ontology Consortium
通讯作者：
The Gene Ontology Consortium
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
DOI：
10.1073/pnas.0505667103
发表时间：
2006-02-07
影响因子：
11.1
作者：
Furihata, T;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie
影响因子：
5.1
作者：
Hellens RP;Allan AC;Friel EN;Bolitho K;Grafton K;Templeton MD;Karunairetnam S;Gleave AP;Laing WA
通讯作者：
Laing WA