Packaging of human immunodeficiency virus type 1 RNA requires cis-acting sequences outside the 5' leader region
Packaging of human immunodeficiency virus type 1 RNA requires cis-acting sequences outside the 5' leader region
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人类免疫缺陷病毒 1 型 RNA 的包装需要 5 前导区外的顺式作用序列
DOI：
10.1128/jvi.67.7.3997-4005.1993
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发表时间：
1993
影响因子：
5.4
通讯作者：
A. Lever
中科院分区：
文献类型：
--
作者：
J. Richardson;L. Child;A. Lever
研究了人类免疫缺陷病毒1型(HIV-1)RNA包埋所需的顺式元件，用一种复制能力强的辅助病毒包装一系列HIV-1载体，并稳定地将其导入人CD4T细胞系。先前在5‘前导区发现的包装信号不足以包裹含有异源基因的小载体。相反，含有额外的Gag和env序列的载体被高效包装，并被转导到滴度超过10(4)cfu/ml的CD4表达的靶细胞中。包含REV反应元件的1.1kb的env基因片段是表达和包装含有顺式作用抑制序列的载体所必需的，并且似乎也包含一个重要的包装信号。该系统成功地包装了小至2.6kb的载体。丰富的、可包装的载体RNA的存在似乎没有干扰野生型HIV-1基因组的包装，这表明在急性感染期间HIV-1RNA的包装容量并不饱和。
cis elements required for the encapsidation of human immunodeficiency virus type 1 (HIV-1) RNA have been investigated by using a replication-competent helper virus to package a series of HIV-1-based vectors which had been stably transfected into human CD4 T-cell lines. A previously identified packaging signal in the 5' leader region was not sufficient for the encapsidation of small vectors containing heterologous genes. In contrast, vectors containing additional gag and env sequences were packaged with high efficiency and transduced into CD4-expressing target cells with titers exceeding 10(4) CFU/ml. The presence of gag sequences did not enhance vector packaging efficiency. A 1.1-kb env gene fragment encompassing the Rev-responsive element was absolutely required for the expression and encapsidation of vectors containing cis-acting repressive sequences and appeared also to contain an important packaging signal. Vectors as small as 2.6 kb were successfully packaged in this system. The presence of abundant, packageable vector RNA did not appear to interfere with encapsidation of the wild-type HIV-1 genome, suggesting that HIV-1 RNA packaging capacity is not saturated during acute infection.
表达氯霉素乙酰转移酶的具有复制能力的人类免疫缺陷病毒（HIV-1）的构建和使用。
DOI：
10.1073/pnas.86.10.3857
发表时间：
1989
影响因子：
11.1
作者：
Terwilliger,EF;Godin,B;Sodroski,JG;Haseltine,WA
通讯作者：
Haseltine,WA
DOI：
10.1073/pnas.85.7.2071
发表时间：
1988-04-01
影响因子：
11.1
作者：
ROSEN, CA;TERWILLIGER, E;HASELTINE, WA
通讯作者：
HASELTINE, WA
影响因子：
56.9
作者：
KOWALSKI, M;POTZ, J;SODROSKI, J
通讯作者：
SODROSKI, J