Evaluation of distinct freezing methods and cryoprotectants for human amniotic fluid stem cells cryopreservation.
Evaluation of distinct freezing methods and cryoprotectants for human amniotic fluid stem cells cryopreservation.
复制标题
人羊水干细胞冷冻保存的不同冷冻方法和冷冻保护剂的评估。
DOI：
10.1155/2012/649353
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发表时间：
2012
影响因子：
--
通讯作者：
Bydlowski SP
中科院分区：
文献类型：
--
作者：
Janz Fde L;Debes Ade A;Cavaglieri Rde C;Duarte SA;Romão CM;Morón AF;Zugaib M;Bydlowski SP
羊水（AF）被描述为生物医学用途间充质干细胞（MSCs）的潜在来源。因此，仍然需要评估能够在冷却后保持这些细胞的活力和干性的替代冷冻保护剂和冷冻方案。对AF干细胞（AFSCs）进行了不同冷冻方法和冷冻保护剂的检测。解冻后评估细胞活力、基因表达、表面标记物和可塑性。AFSCs表达未分化基因Oct4和Nanog；呈现典型的标记（CD29、CD44、CD90和CD105），并能够分化为间充质谱系。所有测试的冷冻保护剂都保留了afsc的特征，但观察到细胞活力的变化。在这方面，二甲亚砜（Me2SO）的效果最好。冷冻方案测试并没有促进AFSCs活力的显著变化。时间程序化和非程序化冷冻方法均可成功保存AFSCs 6个月。虽然测试的冷冻保护剂保持了未分化的基因表达、典型标记和AFSCs的可塑性，但只有Me2SO和甘油具有可行的活力比。
Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing. AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages. All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed. In this concern, dimethyl sulfoxide (Me2SO) showed the best results. The freezing protocols tested did not promote significant changes in the AFSCs viability. Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months. Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios.
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影响因子：
9.8
作者：
Prusa, AR;Marton, E;Hengstschläger, M
通讯作者：
Hengstschläger, M
影响因子：
4.3
作者：
Syme, R;Bewick, M;Glück, S
通讯作者：
Glück, S
DOI：
10.1016/j.bpobgyn.2004.07.001
发表时间：
2004-12-01
影响因子：
5.5
作者：
Fauza, D
通讯作者：
Fauza, D
影响因子：
6.1
作者：
Prusa, AR;Marton, E;Hengstschläger, M
通讯作者：
Hengstschläger, M
影响因子：
6.1
作者：
Moon, Jeong Hee;Lee, Jung Ryeol;Kim, Hae Kwon
通讯作者：
Kim, Hae Kwon