Biomedically relevant circuit-design strategies in mammalian synthetic biology.
Biomedically relevant circuit-design strategies in mammalian synthetic biology.
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DOI：
10.1038/msb.2013.48
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发表时间：
2013
影响因子：
9.9
通讯作者：
中科院分区：
文献类型：
--
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合成生物学的发展和进步是显著的。虽然仍处于起步阶段，但合成生物学在过去十年中取得了很大成就。基因电路设计的改进增加了合成生物学研究的临床应用潜力。最初简单的转录基因开关已经迅速发展成为基于转录、翻译和翻译后调控的各种复杂调控回路。现在使用的诱导剂分子不是具有潜在药理学副作用的化合物，而是人体的代谢物，甚至是天然细胞信号通路的成员。在这篇综述中，我们讨论了哺乳动物合成生物学电路设计的最新进展，并重点介绍了新的设计如何推动合成生物学走向临床实施。解决了光遗传学和细胞间通信的实施方面的开创性研究，特别是光遗传学为临床应用提供了前所未有的机会。沿着合成网络复杂性的增加，多细胞系统现在被用于为下一代电路设计提供平台。
The development and progress in synthetic biology has been remarkable. Although still in its infancy, synthetic biology has achieved much during the past decade. Improvements in genetic circuit design have increased the potential for clinical applicability of synthetic biology research. What began as simple transcriptional gene switches has rapidly developed into a variety of complex regulatory circuits based on the transcriptional, translational and post-translational regulation. Instead of compounds with potential pharmacologic side effects, the inducer molecules now used are metabolites of the human body and even members of native cell signaling pathways. In this review, we address recent progress in mammalian synthetic biology circuit design and focus on how novel designs push synthetic biology toward clinical implementation. Groundbreaking research on the implementation of optogenetics and intercellular communications is addressed, as particularly optogenetics provides unprecedented opportunities for clinical application. Along with an increase in synthetic network complexity, multicellular systems are now being used to provide a platform for next-generation circuit design.
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影响因子：
64.8
作者：
Do, Michael Tri H.;Kang, Shin H.;Xue, Tian;Zhong, Haining;Liao, Hsi-Wen;Bergles, Dwight E.;Yau, King-Wai
通讯作者：
Yau, King-Wai
影响因子：
48
作者：
Bruegmann, Tobias;Malan, Daniela;Sasse, Philipp
通讯作者：
Sasse, Philipp
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
影响因子：
46.9
作者：
Bacchus, William;Lang, Moritz;Fussenegger, Martin
通讯作者：
Fussenegger, Martin
影响因子：
10.5
作者：
Burrill, Devin R.;Inniss, Mara C.;Silver, Pamela A.
通讯作者：
Silver, Pamela A.