Impact of isoflurane anesthesia on D2 receptor occupancy by [18F]fallypride measured by microPET with a modified Logan plot.
Impact of isoflurane anesthesia on D2 receptor occupancy by [18F]fallypride measured by microPET with a modified Logan plot.
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DOI：
10.1002/syn.20955
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发表时间：
2011-11
期刊：
影响因子：
2.3
通讯作者：
Kessler, Robert M.
中科院分区：
文献类型：
--
作者：
Tantawy, Mohammed N.;Peterson, Todd E.;Jones, Carrie K.;Johnson, Kari;Rook, Jerri M.;Conn, P. Jeffrey;Baldwin, Ronald M.;Ansari, M. Sib;Kessler, Robert M.
在先前的工作中，我们报告了一种方法，该方法利用[18 F]fallypride给药后60至120 min收集的成像数据来估计分布体积比DVR′（DVR′ = DVR; DVR = 1 + BPND，其中BPND是受体密度的测量值，在这种情况下为DA D2）。在这项工作中，我们使用这种方法来评估异氟醚麻醉对[18 F]fallypride DVR′的影响。在约1.5%异氟烷下通过尾静脉（第1组）无意识地或通过插入颈静脉（第2组）或尾静脉（第3组）的导管有意识地向大鼠注射[18 F]fallypride。自由进食和饮水约1小时后，用1.5%异氟烷麻醉大鼠，并在microPET中成像60分钟。在[18F]fallypride注射期间，将有意识注射的大鼠（第2组和第3组）置于大鼠限制器中。在实验日之前，使它们习惯于该限制器3天，以最小化限制相关的压力。为了进行比较，对照组大鼠在1.5%异氟烷下静脉注射[18F]氟普利的同时成像120分钟。将60 min采集的DVR′估计值与120 min采集的最后60 min（忽略前60 min后）的DVR′进行比较。此外，使用任意模拟血浆输入函数，用2-组织+血浆隔室模型拟合纹状体时间-活性曲线，以获得60和120 min采集的k3/k4（KIBPND）。异氟烷麻醉导致DVR′估计值显著降低，最高达22%，对照组为15.7 ± 0.3（平均值± SE），第1组为17.7 ± 0.3，第2组为19.2 ± 0.4，第3组为18.8 ± 0.7。隔室模型拟合产生了类似的结果，与60分钟采集相比，120分钟采集的k3/k4降低了约30%（放射性示踪剂的初始有意识吸收）。本研究的结果表明，异氟烷麻醉显著降低大鼠纹状体[18 F]fallypride BPND。同样重要的是，这项工作证明了放射性示踪剂注射后延迟扫描的有效性，并暗示可以用这种方法同时进行不同类型的研究，包括行为和环境对大脑受体影响的研究。
In the previous work, we reported a method that utilized imaging data collected from 60 to 120 min following [18F]fallypride administration to estimate the distribution volume ratio DVR′ (DVR′ ∝ DVR; DVR = 1 + BPND, where BPND is a measure of receptor density, DA D2 in this case). In this work, we use this method to assess the effects of isoflurane anesthesia on [18F]fallypride DVR′. Rats were injected with [18F]fallypride either unconsciously under ~1.5% isoflurane via the tail vein (Group 1) or consciously via a catheter inserted either in the jugular vein (Group 2) or the tail vein (Group 3). After about 1 h of free access to food and water the rats were anesthetized with 1.5% isoflurane and imaged in a microPET for 60 min. The rats that were injected consciously (Groups 2 and 3) were placed in a rat restrainer during [18F]fallypride injection. They were habituated in that restrainer for 3 days prior to the experiment day to minimize restraint-related stress. For comparison, a control group of rats was imaged for 120 min simultaneously with the administration of [18F]fallypride i.v. while under 1.5% isoflurane. The DVR′ estimates from the 60 min acquisitions were compared with the DVR′ from the last 60 min of the 120 min acquisitions (after neglecting the first 60 min). In addition, the striatal time–activity curves were fit with a 2-tissue + plasma compartment model using an arbitrary simulated plasma input function to obtain k3/k4 (≈ BPND) for the 60 and 120 min acquisitions. Isoflurane anesthesia caused a significant reduction, up to 22%, in the DVR′ estimates, which were 15.7 ± 0.3 (mean ± SE) for the controls, 17.7 ± 0.3 for Group 1, 19.2 ± 0.4 for Group 2, and 18.8 ± 0.7 for Group 3. The compartmental model fit produced similar results, ~30% reduction in k3/k4 for the 120-min acquisitions compared with the 60-min acquisitions (initial conscious uptake of the radiotracer). The results of this study demonstrate that isoflurane anesthesia significantly decreases striatal [18F]fallypride BPND in rats. Of similar importance, this work demonstrates the effectiveness of delayed scans following radiotracer injection and the implication that different types of studies can be conducted simultaneously with this method, including studies of behavioral and environmental impact on brain receptors.
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影响因子：
3.1
作者：
Tantawy, Mohammed N.;Jones, Carrie K.;Peterson, Todd E.
通讯作者：
Peterson, Todd E.
影响因子：
7.6
作者：
Riccardi, P;Li, R;Kessler, R
通讯作者：
Kessler, R
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DOI：
10.1523/jneurosci.2559-08.2009
发表时间：
2009-02-11
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
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作者：
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DOI：
10.1007/s00259-003-1171-8
发表时间：
2003-06-01
影响因子：
9.1
作者：
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通讯作者：
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影响因子：
3.3
作者：
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通讯作者：
FILLENZ, M