Imaging mass cytometry reveals tissue-specific cellular immune phenotypes in the mouse knee following ACL injury.
Imaging mass cytometry reveals tissue-specific cellular immune phenotypes in the mouse knee following ACL injury.
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DOI：
10.1016/j.ocarto.2023.100416
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发表时间：
2023-12
期刊：
影响因子：
--
通讯作者：
Griffin, Timothy M.
中科院分区：
文献类型：
--
作者：
South, Sanique M.;Marlin, M. Caleb;Mehta-D'souza, Padmaja;Stephens, Tayte;Conner, Taylor;Burt, Kevin G.;Guthridge, Joel M.;Scanzello, Carla R.;Griffin, Timothy M.
开发一种成像质谱流式细胞术方法，用于识别小鼠膝关节在无创性压迫损伤后滑膜和髌下脂肪垫（IFP）中的复杂细胞表型、细胞间相互作用以及细胞群变化。
15只雄性C57BL/6小鼠在8周龄时开始喂食高脂饮食，持续8周，然后在24周龄时随机分为假手术组、0.88毫米或1.7毫米膝关节压缩位移组。加载2周后，对四肢进行组织学和成像质谱流式细胞术分析准备，重点关注滑膜和IFP中的髓系免疫细胞群。
1.7毫米压缩导致前交叉韧带（ACL）断裂、创伤后骨关节炎的发生，并且与假手术组或0.88毫米压缩组相比，滑膜和IFP组织的细胞数量增加了2 - 3倍。成像质谱流式细胞术在滑膜中识别出11个髓系细胞亚群，在IFP中识别出7个，其中大约一半在ACL损伤2周后与脉管系统相关联而升高。值得注意的是，两个单核细胞/巨噬细胞亚群和一个MHC II高表达群体在损伤后2周在滑膜中升高，但在IFP中没有。血管和免疫细胞的相互作用在滑膜中尤其多样，包含5个髓系细胞群的8种独特组合，包括一个单核细胞/巨噬细胞群、一个MHC II高表达群体以及3个不同的未定义的F4/80阳性髓系群体。
为小鼠开发一种成像质谱流式细胞术方法使我们能够识别出滑膜和IFP中与血管相关的多种髓系细胞亚群。这些亚群在损伤后2周在滑膜和IFP组织中升高情况不同，为组织特异性免疫调节提供了新的细节。
To develop an imaging mass cytometry method for identifying complex cell phenotypes, inter-cellular interactions, and population changes in the synovium and infrapatellar fat pad (IFP) of the mouse knee following a non-invasive compression injury. Fifteen male C57BL/6 mice were fed a high-fat diet for 8 weeks prior to random assignment to sham, 0.88 mm, or 1.7 mm knee compression displacement at 24 weeks of age. 2-weeks after loading, limbs were prepared for histologic and imaging mass cytometry analysis, focusing on myeloid immune cell populations in the synovium and IFP. 1.7 mm compression caused anterior cruciate ligament (ACL) rupture, development of post-traumatic osteoarthritis, and a 2- to 3-fold increase in cellularity of synovium and IFP tissues compared to sham or 0.88 mm compression. Imaging mass cytometry identified 11 myeloid cell subpopulations in synovium and 7 in IFP, of which approximately half were elevated 2 weeks after ACL injury in association with the vasculature. Notably, two monocyte/macrophage subpopulations and an MHC IIhi population were elevated 2-weeks post-injury in the synovium but not IFP. Vascular and immune cell interactions were particularly diverse in the synovium, incorporating 8 unique combinations of 5 myeloid cell populations, including a monocyte/macrophage population, an MHC IIhi population, and 3 different undefined F4/80+ myeloid populations. Developing an imaging mass cytometry method for the mouse enabled us to identify a diverse array of synovial and IFP vascular-associated myeloid cell subpopulations. These subpopulations were differentially elevated in synovial and IFP tissues 2-weeks post injury, providing new details on tissue-specific immune regulation.
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影响因子：
4.4
作者：
Ramezanpour, Sara;Kanthawang, Thanat;Joseph, Gabby B.
通讯作者：
Joseph, Gabby B.
影响因子：
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Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
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39.2
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7.3
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Haraden, Collin A.;Huebner, Janet L.;Kraus, Virginia Byers
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