Both CD4⁺ and CD8⁺ lymphocytes participate in the IFN-γ response to filamentous hemagglutinin from Bordetella pertussis in infants, children, and adults.
Both CD4⁺ and CD8⁺ lymphocytes participate in the IFN-γ response to filamentous hemagglutinin from Bordetella pertussis in infants, children, and adults.
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DOI：
10.1155/2012/795958
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发表时间：
2012
影响因子：
--
通讯作者：
Mascart F
中科院分区：
文献类型：
--
作者：
Dirix V;Verscheure V;Vermeulen F;De Schutter I;Goetghebuer T;Locht C;Mascart F
婴儿 CD4+ T 细胞对细菌感染或疫苗的反应已得到广泛研究，而 CD8+ T 细胞反应的研究主要集中在病毒和细胞内寄生虫感染上。在此，我们研究了婴儿、儿童和成人百日咳博德特氏菌感染以及婴儿百日咳疫苗接种后 CD8+ T 细胞的反应。循环淋巴细胞丝状血凝素特异性 IFN-γ 分泌被抗 MHC-I 或 MHC-II 抗体阻断，表明 CD4+ 和 CD8+ T 淋巴细胞参与 IFN-γ 的产生。流式细胞术分析证实，两种细胞类型均合成抗原特异性 IFN-γ，尽管 CD4+ 淋巴细胞是该细胞因子的主要来源。 CD8+ 细胞的 IFN-γ 合成依赖于 CD4+ T 细胞，选择性耗竭实验证明了这一点。此外，CD4+细胞合成IFN-γ有时会被CD8+淋巴细胞抑制，表明CD8+调节性T细胞的存在。 CD4+ 和 CD8+ T 淋巴细胞双重分泌 IFN-γ 在百日咳中的作用仍有待研究。
Infant CD4+ T-cell responses to bacterial infections or vaccines have been extensively studied, whereas studies on CD8+ T-cell responses focused mainly on viral and intracellular parasite infections. Here we investigated CD8+ T-cell responses upon Bordetella pertussis infection in infants, children, and adults and pertussis vaccination in infants. Filamentous hemagglutinin-specific IFN-γ secretion by circulating lymphocytes was blocked by anti-MHC-I or -MHC-II antibodies, suggesting that CD4+ and CD8+ T lymphocytes are involved in IFN-γ production. Flow cytometry analyses confirmed that both cell types synthesized antigen-specific IFN-γ, although CD4+ lymphocytes were the major source of this cytokine. IFN-γ synthesis by CD8+ cells was CD4+ T cell dependent, as evidenced by selective depletion experiments. Furthermore, IFN-γ synthesis by CD4+ cells was sometimes inhibited by CD8+ lymphocytes, suggesting the presence of CD8+ regulatory T cells. The role of this dual IFN-γ secretion by CD4+ and CD8+ T lymphocytes in pertussis remains to be investigated.
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影响因子：
4.3
作者：
FOA, R;GIUBELLINO, MC;FERRANDO, ML
通讯作者：
FERRANDO, ML
影响因子：
20.3
作者：
Hermann, E;Truyens, C;Carlier, Y
通讯作者：
Carlier, Y
影响因子：
3.7
作者：
Elrefaei, Mohamed;Burke, Candace M.;Cao, Huyen
通讯作者：
Cao, Huyen
影响因子：
5.8
作者：
Bourreau, Eliane;Ronet, Catherine;Launois, Pascal
通讯作者：
Launois, Pascal
影响因子：
15.3
作者：
Leef, M;Elkins, KL;Shahin, RD
通讯作者：
Shahin, RD