Expression of progesterone receptor in human keratinocytes.
Expression of progesterone receptor in human keratinocytes.
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DOI：
10.3346/jkms.2000.15.6.647
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发表时间：
2000-12
影响因子：
4.5
通讯作者：
Kang WH
中科院分区：
文献类型：
--
作者：
Im S;Lee ES;Kim W;Song J;Kim J;Lee M;Kang WH
尽管人类皮肤对女性性激素的反应多种多样，但人类皮肤中雌激素和孕激素的细胞和亚细胞靶点以及作用机制尚不清楚。皮肤中雌激素受体（ER）和孕激素受体（PR）的检测对了解雌激素和孕激素的作用具有重要意义。在人角质形成细胞原代培养中，采用免疫细胞化学和逆转录聚合酶链反应（RT-PCR）检测ER和PR的表达。免疫组化法对石蜡包埋的皮肤组织进行人ER和PR单克隆抗体染色。培养的人角质形成细胞表达细胞质PR蛋白和PR mRNA转录物。相比之下，ER仅在mRNA水平上检测到。瘙痒性荨麻疹丘疹、妊娠斑块（PUPPP）和牛皮癣样品的基底上角质细胞仅PR阳性，结节性红斑样品的ER和PR均阴性。PUPPP的病变表皮显示PR阳性免疫反应，而非病变表皮则没有。正常人皮肤中没有其他细胞被ER和PR染色。本研究表明，通过表达PR，人角质形成细胞可作为孕酮作用的靶点。
Despite the various responses of human skin to female sex hormones, cellular and subcellular targets and the mechanisms of action of estrogen and progesterone in human skin are not well understood. The detection of estrogen receptor (ER) and progesterone receptor (PR) in the skin is of great importance to understand the effect of estrogen and progesterone. In primary cultures of human keratinocytes, expression of ER and PR was monitored by immunocytochemistry and reverse transcriptase polymerase chain reaction (RT-PCR). Paraffin embedded skin tissues were stained with monoclonal antibodies to human ER and PR by immunohistochemistry. Cultured human keratinocytes expressed cytoplasmic PR protein and PR mRNA transcripts. By contrast, ER was detected only at the mRNA level. Suprabasal keratinocytes from samples of pruritic urticarial papules, plaques of pregnancy (PUPPP) and psoriasis were stained positively only for PR, while those from samples of erythema nodosum were negative for both ER and PR. Lesional epidermis of PUPPP showed positive PR immunoreactivity, while nonlesional epidermis did not. No other cells in the normal human skin were stained with ER and PR. The present study suggests that by expressing PR human keratinocytes act as targets for progesterone action.
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DOI：
10.1016/0960-0760(95)00091-d
发表时间：
1995-06-01
影响因子：
4.1
作者：
EDWARDS, DP;ALTMANN, M;BECK, CA
通讯作者：
BECK, CA
DOI：
10.1016/s0190-9622(81)70071-9
发表时间：
1981-01-01
影响因子：
13.8
作者：
SANCHEZ, NP;PATHAK, MA;MIHM, MC
通讯作者：
MIHM, MC
DOI：
10.1016/0006-291x(87)91416-1
发表时间：
1987-03-13
影响因子：
3.1
作者：
MISRAHI, M;ATGER, M;MILGROM, E
通讯作者：
MILGROM, E
DOI：
10.1016/0002-9378(83)90752-4
发表时间：
1983-01-01
影响因子：
9.8
作者：
GAO, YL;TWIGGS, LB;PREM, KA
通讯作者：
PREM, KA
DOI：
10.1111/j.1471-0528.1998.tb10056.x
发表时间：
1998-02-01
期刊：
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY
影响因子：
--
作者：
Hodgins, MB;Spike, RC;MacLean, AB
通讯作者：
MacLean, AB