In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data.
In vitro radiosensitivity of tumour cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data.
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肿瘤细胞和颈部癌的肿瘤细胞和成纤维细胞的体外放射敏性：相互关系以及与临床数据的相关性。
DOI：
10.1038/sj.bjc.6690172
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发表时间：
1999-03
影响因子：
8.8
通讯作者：
Overgaard, J
中科院分区：
文献类型：
--
作者：
Stausbol-Gron, B;Bentzen, SM;Jorgensen, KE;Nielsen, OS;Bundgaard, T;Overgaard, J
关键词：
其目的是表征细胞在体外放射敏感性的变化在鳞状细胞癌的头部和颈部，并测试不同的放射敏感性和临床和组织病理学数据的措施之间可能存在的相关性。使用改良Courtenay-Mills软琼脂克隆形成试验结合免疫细胞化学分析，对71例患者的肿瘤活检组织进行了细胞体外放射敏感性评估。放射敏感性定量为2戈伊辐射剂量后的存活分数，与细胞类型无关（总体SF 2），或基于细胞类型的鉴定（肿瘤细胞SF 2、成纤维细胞SF 2）。63例活检来自原发肿瘤，8例来自复发肿瘤。总体接种效率范围为0.005 - 1.60%，中位数为0.052%。从活检中获得的大多数集落为成纤维细胞标记阳性;肿瘤标记阳性集落的比例范围为1 - 88%，中位数为15%。中位总体SF 2为0.47（范围0.24-0.96），中位肿瘤细胞SF 2为0.50（范围0.11-1.0），中位成纤维细胞SF 2为0.49（范围0.24-1.0）。比较来自独立实验的数据，总体SF 2与成纤维细胞的SF 2显著相关（2 P = 0.006），但与肿瘤细胞SF 2不相关。在同一个体中测量的肿瘤细胞和成纤维细胞放射敏感性不相关（r = 0.06，95%CI [-0.19，0.30]）。这一发现似乎排除了肿瘤细胞和成纤维细胞的放射敏感性之间的强相关性。关于临床特征，肿瘤放射敏感性的措施都与T-和N-类别，阶段，肿瘤大小，性别和年龄。但肿瘤细胞放射敏感性随组织分化程度的增加而降低（2 P = 0.012）。在对同一患者材料的两项独立分析中发现了相同的趋势。在整体SF 2或成纤维细胞SF 2的情况下，这种相关性不显著。© 1999癌症研究运动
The aim was to characterize the variation in the cellular in vitro radiosensitivities in squamous cell carcinomas of the head and neck, and to test for a possible correlation between different measures of radiosensitivity and the clinical and histopathological data. Cellular in vitro radiosensitivities were assessed in tumour biopsies from 71 patients using the modified Courtenay–Mills soft agar clonogenic assay combined with an immunocytochemical analysis. Radiosensitivity was quantified as the surviving fraction after a radiation dose of 2 Gy irrespective of cell type (overall SF2), or based on identification of cell type (tumour cell SF2, fibroblast SF2). Sixty-three biopsies were from primary tumours, and eight were from recurrences. Overall plating efficiency ranged from 0.005 to 1.60% with a median of 0.052%. The majority of the colonies obtained from the biopsies were fibroblast marker-positive; the proportion of tumour marker-positive colonies ranged from 1 to 88% with a median of 15%. The median overall SF2 was 0.47 (range 0.24–0.96), the median tumour cell SF2 was 0.50 (range 0.11–1.0) and the median fibroblast SF2 was 0.49 (range 0.24–1.0). Comparing data from independent experiments, the overall SF2 was significantly correlated with the SF2 of fibroblasts (2P = 0.006) but not with the tumour cell SF2. The tumour cell and fibroblast radiosensitivities measured in the same individuals were not correlated (r = 0.06, 95% CI [–0.19, 0.30]). This finding seems to preclude a strong correlation between the radiosensitivity of tumour cells and fibroblasts. Concerning the clinical characteristics, neither of the measures of tumour radiosensitivity was correlated with T- and N-category, stage, tumour size, sex and age. However, the tumour cell radiosensitivity decreased with increasing grade of histopathological differentiation (2P = 0.012). The same tendency was found in two independent analyses of the same patient material. This correlation was not significant in case of the overall SF2 or the fibroblast SF2. © 1999 Cancer Research Campaign
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DOI：
10.1016/0360-3016(81)90377-1
发表时间：
1981-01-01
影响因子：
7
作者：
FERTIL, B;MALAISE, EP
通讯作者：
MALAISE, EP
DOI：
10.1016/s0360-3016(97)00509-9
发表时间：
1997-11-01
影响因子：
7
作者：
Eschwege, F;Bourhis, J;Luboinski, B
通讯作者：
Luboinski, B
DOI：
10.1016/0360-3016(94)90350-6
发表时间：
1994-11-15
影响因子：
7
作者：
GIRINSKY, T;BERNHEIM, A;FERTIL, B
通讯作者：
FERTIL, B
影响因子：
3.5
作者：
AZUMI, N;BATTIFORA, H
通讯作者：
BATTIFORA, H
DOI：
10.1001/jama.1920.02620100016007
发表时间：
1920-01-01
影响因子：
--
作者：
Broders, AC
通讯作者：
Broders, AC