Circular RNA CircSLC8A1 contributes to osteogenic differentiation in hBMSCs via CircSLC8A1/miR-144-3p/RUNX1 in periprosthetic osteolysis.
Circular RNA CircSLC8A1 contributes to osteogenic differentiation in hBMSCs via CircSLC8A1/miR-144-3p/RUNX1 in periprosthetic osteolysis.
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DOI：
10.1111/jcmm.17633
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发表时间：
2023-01
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
--
作者：
Circular RNAs (circRNAs) are often found in eukaryocyte and have a role in the pathogenesis of a variety of human disorders. Our related research has shown the differential expression of circRNAs in periprosthetic osteolysis (PPOL). However, the involvement of circRNAs in the exact process is yet unknown. CircSLC8A1 expression was evaluated in clinical samples and human bone marrow mesenchymal stem cells (hBMSCs) in this investigation using quantitative real‐time PCR. In vitro and in vivo studies were conducted to explicate its functional role and pathway. We demonstrated CircSLC8A1 is involved in PPOL using gain‐ and loss‐of‐function methods. The association of CircSLC8A1 and miR‐144‐3p, along with miR‐144‐3p and RUNX1, was predicted using bioinformatics. RNA pull‐down and luciferase assays confirmed it. The impact of CircSLC8A1 in the PPOL‐mouse model was also investigated using adeno‐associated virus. CircSLC8A1 was found to be downregulated in PPOL patients' periprosthetic tissues. Overexpression of CircSLC8A1 promoted osteogenic differentiation (OD) and inhibited apoptosis of hBMSCs in vitro. The osteogenic markers of RUNX1, osteopontin (OPN) and osteocalcin (OCN) were significantly upregulated in hBMSCs after miR‐144‐3p inhibitor was transferred. Mechanistic analysis demonstrated that CircSLC8A1 directly bound to miR‐144‐3p and participated in PPOL through the miR‐144‐3p/RUNX1 pathway in hBMSCs. Micro‐CT and quantitative analysis showed that CircSLC8A1 markedly inhibited PPOL, and osteogenic markers (RUNX1, OPN and OCN) were significantly increased (P<0.05) in the mice model. Our findings prove that CircSLC8A1 exerted a regulatory role in promoting osteogenic differentiation in hBMSCs, and CircSLC8A1/miR‐144‐3p/RUNX1 pathway may provide a potential target for prevention of PPOL.
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影响因子：
3.6
作者：
Ni S;Jiang T;Hao S;Luo P;Wang P;Almatari Y;Wang Y;Zhang Z;Guo L
通讯作者：
Guo L
影响因子：
0.9
作者：
Mitra A;Pfeifer K;Park KS
通讯作者：
Park KS
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影响因子：
21.3
作者：
Harland LTG;Simon CS;Senft AD;Costello I;Greder L;Imaz-Rosshandler I;Göttgens B;Marioni JC;Bikoff EK;Porcher C;de Bruijn MFTR;Robertson EJ
通讯作者：
Robertson EJ
影响因子：
4.6
作者：
Fitch, Simon R.;Kapeni, Chrysa;Ottersbach, Katrin
通讯作者：
Ottersbach, Katrin
影响因子：
4.2
作者：
Jiang, Yasu;Cai, Yunhui;Feng, Shichun
通讯作者：
Feng, Shichun