Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
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拟南芥卵形家族蛋白是一种新型转录阻遏蛋白家族，控制植物生长和发育的多个方面
DOI：
10.1371/journal.pone.0023896
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Chen JG
中科院分区：
文献类型：
--
作者：
Wang S;Chang Y;Guo J;Zeng Q;Ellis BE;Chen JG
研究背景拟南芥基因组包含18个编码Ovate家族蛋白（AtOFPs）的基因，AtOFPs是一个以保守的OVATE结构域为特征的蛋白家族，该结构域由大约70个氨基酸组成，最初在番茄OVATE蛋白中发现。在AtOFP家族成员中，AtOFP 1已显示部分通过抑制AtGA 20 ox 1的表达来抑制细胞伸长，AtOFP 4已显示通过与KNOTTED 1样同源域蛋白7（KNAT 7）相互作用来调节次生细胞壁形成，AtOFP 5已显示在早期胚囊发育期间调节BEL 1样同源域1（BLH 1）-KNAT 3复合物的活性，但对其他AtOFPs的功能知之甚少。方法/主要发现我们在这里证明，AtOFP蛋白可以作为有效的转录抑制剂在拟南芥原生质体瞬时表达系统。对AtOFP功能缺失等位基因的分析表明，AtOFP基因在调控植物生长发育方面可能具有重叠功能，因为所鉴定的单个突变体，包括AtOFP 1、AtOFP 4、AtOFP 8、AtOFP 10、AtOFP 15和AtOFP 16中的T-DNA插入突变体，均未表现出明显的形态缺陷。此外，Atofp 1、Atofp 4和Atofp 15、Atofp 16双突变体与野生型仍然没有显著差异。另一方面，过量表达AtOFP基因的植物表现出多种异常表型，可分为三类，表明AtOFP基因在调节植物生长发育中可能具有多种功能。进一步的分析表明，AtOFP 1以胚后方式调节子叶发育，全球转录谱显示它抑制许多其他基因的表达。结论/意义我们的研究结果表明AtOFPs作为转录抑制因子发挥作用，它们调节植物生长和发育的多个方面。这些结果提供了一个以前未知的转录抑制因子家族的第一个概述，并揭示了它们在植物生长发育中的可能作用。
Background The Arabidopsis genome contains 18 genes that are predicted to encode Ovate Family Proteins (AtOFPs), a protein family characterized by a conserved OVATE domain, an approximately 70-amino acid domain that was originally found in tomato OVATE protein. Among AtOFP family members, AtOFP1 has been shown to suppress cell elongation, in part, by suppressing the expression of AtGA20ox1, AtOFP4 has been shown to regulate secondary cell wall formation by interact with KNOTTED1-LIKE HOMEODOMAIN PROTEIN 7 (KNAT7), and AtOFP5 has been shown to regulate the activity of a BEL1-LIKEHOMEODOMAIN 1(BLH1)-KNAT3 complex during early embryo sac development, but little is known about the function of other AtOFPs. Methodology/Principal Findings We demonstrated here that AtOFP proteins could function as effective transcriptional repressors in the Arabidopsis protoplast transient expression system. The analysis of loss-of-function alleles of AtOFPs suggested AtOFP genes may have overlapping function in regulating plant growth and development, because none of the single mutants identified, including T-DNA insertion mutants in AtOFP1, AtOFP4, AtOFP8, AtOFP10, AtOFP15 and AtOFP16, displayed any apparent morphological defects. Further, Atofp1 Atofp4 and Atofp15 Atofp16 double mutants still did not differ significantly from wild-type. On the other hand, plants overexpressing AtOFP genes displayed a number of abnormal phenotypes, which could be categorized into three distinct classes, suggesting that AtOFP genes may also have diverse functions in regulating plant growth and development. Further analysis suggested that AtOFP1 regulates cotyledon development in a postembryonic manner, and global transcript profiling revealed that it suppress the expression of many other genes. Conclusions/Significance Our results showed that AtOFPs function as transcriptional repressors and they regulate multiple aspects of plant growth and development. These results provided the first overview of a previously unknown transcriptional repressor family, and revealed their possible roles in plant growth and development.
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影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
5.1
作者：
Liscum, E;Reed, JW
通讯作者：
Reed, JW
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1073/pnas.0501181102
发表时间：
2005-03-29
影响因子：
11.1
作者：
Hackbusch, J;Richter, K;Uhrig, JF
通讯作者：
Uhrig, JF
影响因子：
11.6
作者：
Tiwari, SB;Hagen, G;Guilfoyle, T
通讯作者：
Guilfoyle, T