Comparative transcriptomics analysis reveals difference of key gene expression between banana and plantain in response to cold stress.
Comparative transcriptomics analysis reveals difference of key gene expression between banana and plantain in response to cold stress.
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比较转录组学分析揭示香蕉和车前草响应冷胁迫的关键基因表达差异
DOI：
10.1186/s12864-015-1551-z
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发表时间：
2015-06-10
期刊：
影响因子：
4.4
通讯作者：
Yi GJ
中科院分区：
文献类型：
--
作者：
Yang QS;Gao J;He WD;Dou TX;Ding LJ;Wu JH;Li CY;Peng XX;Zhang S;Yi GJ
背景：香蕉和大蕉（芭蕉属）是全球数百万人饮食的重要组成部分。低温是影响全球香蕉生产的主要环境胁迫之一。为了解车前草耐冷性的分子机制，本研究采用RNA-Seq方法对受冷胁迫0、3和6 h的冷敏感型香蕉和耐寒型车前草进行了转录组学比较分析。结果表明，大蕉在低温胁迫3 h和6 h分别鉴定出10个和68个差异表达基因，香蕉在低温胁迫下分别鉴定出40个和238个差异表达基因。氧化石墨烯分类分析表明，在香蕉和大蕉中发现的大部分deg都属于转录调控、应激信号转导响应等11类。在6小时的冷胁迫下，香蕉和大蕉具有相似的28℃特征，这表明它们在应对冷胁迫时具有一些共同的适应过程。在耐寒大蕉中发现了17个独特的deg，它们参与了信号转导、非生物胁迫、铜离子平衡、光合作用和光呼吸、糖刺激、蛋白质修饰等。通过延长实验时间（0、3、6、24和48 h），筛选到ice1和mybs3等12个早期应答基因，通过qPCR方法进行鉴定和确认，发现冷敏香蕉和耐寒大蕉对冷胁迫的应答关键基因，尤其是ice1和mybs3的表达差异显著。结论大车前草在不同阶段的冷胁迫下，通过调控office1和mybs3的表达，选择性激活了耐寒途径。我们认为，车前草中ICE1和MYBS3耐冷途径的快速激活和选择性诱导以及其他冷特异性基因的表达可能是车前草比香蕉具有更高抗寒性的主要原因之一。
BackgroundBanana and plantain (Musa spp.) comprise an important part of diets for millions of people around the globe. Low temperature is one of the key environmental stresses which greatly affects the global banana production. To understand the molecular mechanism of the cold-tolerance in plantain we used RNA-Seq based comparative transcriptomics analyses for both cold-sensitive banana and cold-tolerant plantain subjected to the cold stress for 0, 3 and 6 h.ResultsThe cold-response genes at early stage are identified and grouped in both species by GO analysis. The results show that 10 and 68 differentially expressed genes (DEGs) are identified for 3 and 6 h of cold stress respectively in plantain, while 40 and 238 DEGs are identified respectively in banana. GO classification analyses show that the majority of DEGs identified in both banana and plantain belong to 11 categories including regulation of transcription, response to stress signal transduction, etc. A similar profile for 28 DEGs was found in both banana and plantain for 6 h of cold stress, suggesting both share some common adaptation processes in response to cold stress. There are 17 DEGs found uniquely in cold-tolerance plantain, which were involved in signal transduction, abiotic stress, copper ion equilibrium, photosynthesis and photorespiration, sugar stimulation, protein modifications etc. Twelve early responsive genes includingICE1andMYBS3were selected and further assessed and confirmed by qPCR in the extended time course experiments (0, 3, 6, 24 and 48 h), which revealed significant expression difference of key genes in response to cold stress, especiallyICE1andMYBS3between cold-sensitive banana and cold-tolerant plantain.ConclusionsWe found that the cold-tolerance pathway appears selectively activated by regulation ofICE1andMYBS3expression in plantain under different stages of cold stress.We conclude that the rapid activation and selective induction of ICE1 and MYBS3 cold tolerance pathways in plantain,along with expression of other cold-specific genes, may be one of the main reasons that plantain has higher cold resistance than banana.
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5.6
作者：
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通讯作者：
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