Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro.
Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro.
复制标题
DOI：
10.1038/s41598-018-25113-4
复制
发表时间：
2018-04-30
影响因子：
4.6
通讯作者：
Donahue HJ
中科院分区：
文献类型：
--
作者：
Meng F;Murray GF;Kurgan L;Donahue HJ
骨对机械负荷的合成代谢反应部分是骨细胞对流体流动引起的剪切应力的反应的结果。了解暴露于流体流的骨细胞中激活的信号通路可以识别参与骨骼对机械负荷响应的新信号通路。生物信息学提供了独特的视角，并为理解这些信号通路提供了关键的第一步。我们检查了小鼠骨细胞 MLO-Y4 细胞中响应流体流动而差异表达的基因编码的蛋白质。我们考虑了结构和功能特征，包括假定的内在无序、进化保守、蛋白质-蛋白质相互作用网络的互连性以及细胞定位。我们的分析表明，由流体流动激活基因编码的蛋白质具有低于预期的保守性，在内在紊乱中被耗尽，维持小鼠蛋白质组的典型连接水平，并且在细胞质和细胞外空间中发现。通路分析表明，这些蛋白质与细胞对应激、趋化因子和细胞因子活性、酶结合和破骨细胞分化的反应有关。流激活基因编码的蛋白质紊乱程度低于预期，表明它们相对特化。
The anabolic response of bone to mechanical load is partially the result of osteocyte response to fluid flow-induced shear stress. Understanding signaling pathways activated in osteocytes exposed to fluid flow could identify novel signaling pathways involved in the response of bone to mechanical load. Bioinformatics allows for a unique perspective and provides key first steps in understanding these signaling pathways. We examined proteins encoded by genes differentially expressed in response to fluid flow in murine osteocytic MLO-Y4 cells. We considered structural and functional characteristics including putative intrinsic disorder, evolutionary conservation, interconnectedness in protein-protein interaction networks, and cellular localization. Our analysis suggests that proteins encoded by fluid flow activated genes have lower than expected conservation, are depleted in intrinsic disorder, maintain typical levels of connectivity for the murine proteome, and are found in the cytoplasm and extracellular space. Pathway analyses reveal that these proteins are associated with cellular response to stress, chemokine and cytokine activity, enzyme binding, and osteoclast differentiation. The lower than expected disorder of proteins encoded by flow activated genes suggests they are relatively specialized.
登录
查看更多内容
影响因子：
4.1
作者：
Jung SM;Kim KW;Yang CW;Park SH;Ju JH
通讯作者：
Ju JH
影响因子：
1.6
作者：
Howell, Mark;Green, Ryan;Uversky, Vladimir N.
通讯作者：
Uversky, Vladimir N.
影响因子：
2.4
作者：
Govey, Peter M.;Kawasawa, Yuka Imamura;Donahue, Henry J.
通讯作者：
Donahue, Henry J.
影响因子：
5.8
作者：
Dosztányi, Z;Csizmok, V;Simon, I
通讯作者：
Simon, I
影响因子：
4.5
作者：
He X;Zhang J
通讯作者：
Zhang J