Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha.
Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha.
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DOI：
10.1371/journal.pone.0204964
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Nakagawa T
中科院分区：
文献类型：
--
作者：
Mano S;Nishihama R;Ishida S;Hikino K;Kondo M;Nishimura M;Yamato KT;Kohchi T;Nakagawa T
地茅（Marchantia polymorpha）是一种新兴的基系植物研究模式种。本研究构建了两个Gateway克隆兼容的二元载体系列R4pMpGWB和R4L1pMpGWB，以促进转基因多态m.a的产生。R4pMpGWB系列允许任何启动子和任何具有特定报告子或标签的编码序列的三方重组。R4pMpGWB系列的报告/标记物有GUS、ELuc（PEST）、FLAG、3×HA、4×Myc、mRFP1、Citrine、mCitrine、er靶向mCitrine和核靶向mCitrine。R4L1pMpGWB系列适用于启动子分析。R4L1pMpGWB载体结构与R4pMpGWB载体相同，不同之处在于attR2位点被attL1取代，使得任何带有报告子或标签的启动子都可以进行二部重组。R4L1pMpGWB系列的报告/标记有GUS、G3GFP-GUS、LUC、ELuc（PEST）、Citrine、mCitrine、ER-targeted mCitrine和mCitrine- nls。两个载体系列在多形霉细胞中均能正常表达。这些载体将有助于设计和组装质粒构建和转基因多态霉的产生。
The liverwort Marchantia polymorpha is an emerging model species for basal lineage plant research. In this study, two Gateway cloning-compatible binary vector series, R4pMpGWB and R4L1pMpGWB, were generated to facilitate production of transgenic M. polymorpha. The R4pMpGWB series allows tripartite recombination of any promoter and any coding sequence with a specific reporter or tag. Reporters/tags for the R4pMpGWB series are GUS, ELuc(PEST), FLAG, 3×HA, 4×Myc, mRFP1, Citrine, mCitrine, ER-targeted mCitrine and nucleus-targeted mCitrine. The R4L1pMpGWB series is suitable for promoter analysis. R4L1pMpGWB vector structure is the same as that of R4pMpGWB vectors, except that the attR2 site is replaced with attL1, enabling bipartite recombination of any promoter with a reporter or tag. Reporters/tags for the R4L1pMpGWB series are GUS, G3GFP-GUS, LUC, ELuc(PEST), Citrine, mCitrine, ER-targeted mCitrine and mCitrine-NLS. Both vector series were functional in M. polymorpha cells. These vectors will facilitate the design and assembly of plasmid constructs and generation of transgenic M. polymorpha.
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影响因子：
3.7
作者：
Aboulela M;Tanaka Y;Nishimura K;Mano S;Nishimura M;Ishiguro S;Kimura T;Nakagawa T
通讯作者：
Nakagawa T
影响因子：
3.7
作者：
Ishizaki K;Nishihama R;Ueda M;Inoue K;Ishida S;Nishimura Y;Shikanai T;Kohchi T
通讯作者：
Kohchi T
影响因子：
4.9
作者：
Kamada, T;Nito, K;Nishimura, M
通讯作者：
Nishimura, M
影响因子：
1.6
作者：
Hino, Takeshi;Tanaka, Yuji;Nakagawa, Tsuyoshi
通讯作者：
Nakagawa, Tsuyoshi
影响因子：
3.7
作者：
GAMBORG, OL;MILLER, RA;OJIMA, K
通讯作者：
OJIMA, K