Characterization of the Biophysical Properties and Cell Adhesion Interactions of Marine Invertebrate Collagen from Rhizostoma pulmo.
Characterization of the Biophysical Properties and Cell Adhesion Interactions of Marine Invertebrate Collagen from Rhizostoma pulmo.
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胶原蛋白是动物界中最普遍存在的生物大分子，通常用作再生医学疗法和生物医学研究中的生物材料。在这些应用中使用的胶原蛋白通常来源于哺乳动物来源，由于广泛的宗教限制，对动物权利的道德关注日益增加以及人畜共患病传播的持续风险，这带来了社会学问题。这些问题导致越来越多的研究替代胶原蛋白来源，其中海洋胶原蛋白，特别是来自水母，已经成为一种有前途的资源。本研究提供了一个表征的生物物理特性和细胞粘附的相互作用的胶原蛋白来自水母Rupstoma pulmo（JCol）。用圆二色光谱和原子力显微镜观察了JCol的三螺旋构象和纤维形态。还使用肝素亲和层析来证明JCol与固定化肝素结合的能力。使用整联蛋白阻断抗体和HT-1080人纤维肉瘤细胞的细胞粘附试验显示，与JCol的粘附主要通过β1整联蛋白进行，α2β1整联蛋白除外。还显示硫酸乙酰肝素结合在成纤维细胞和间充质基质细胞粘附至JCol中比I型哺乳动物胶原（大鼠尾胶原）起更大的作用。总的来说，这项研究突出了来自哺乳动物和水母来源的胶原蛋白之间的相似性和差异，在利用替代胶原蛋白来源进行生物医学研究时应考虑这些差异。
Collagen is the most ubiquitous biomacromolecule found in the animal kingdom and is commonly used as a biomaterial in regenerative medicine therapies and biomedical research. The collagens used in these applications are typically derived from mammalian sources which poses sociological issues due to widespread religious constraints, rising ethical concern over animal rights and the continuous risk of zoonotic disease transmission. These issues have led to increasing research into alternative collagen sources, of which marine collagens, in particular from jellyfish, have emerged as a promising resource. This study provides a characterization of the biophysical properties and cell adhesion interactions of collagen derived from the jellyfish Rhizostoma pulmo (JCol). Circular dichroism spectroscopy and atomic force microscopy were used to observe the triple-helical conformation and fibrillar morphology of JCol. Heparin-affinity chromatography was also used to demonstrate the ability of JCol to bind to immobilized heparin. Cell adhesion assays using integrin blocking antibodies and HT-1080 human fibrosarcoma cells revealed that adhesion to JCol is primarily performed via β1 integrins, with the exception of α2β1 integrin. It was also shown that heparan sulfate binding plays a much greater role in fibroblast and mesenchymal stromal cell adhesion to JCol than for type I mammalian collagen (rat tail collagen). Overall, this study highlights the similarities and differences between collagens from mammalian and jellyfish origins, which should be considered when utilizing alternative collagen sources for biomedical research.
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